Şevik Murat
Department of Molecular Microbiology, Veterinary Control Institute, Meram, 42080 Konya, Turkey.
Virusdisease. 2017 Sep;28(3):345-348. doi: 10.1007/s13337-017-0392-6. Epub 2017 Aug 22.
Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and can cause skin disease in sheep and goats. In this study, two outbreaks of ORFV infection in goats in the Central Anatolian region of Turkey were investigated. Samples were collected from 1- to 4-month-old kids (n = 9) in two different flocks in the Aksaray and Konya Provinces during the months of March and May 2016. The presence of ORFV in suspected samples was confirmed by PCR using primers specific to envelope gene (B2L). The analysis of the B2L gene sequences revealed that the nucleotide homology between the two isolates in the present study was 100%, whereas the similarity with Parapoxvirus isolates from different regions ranged from 83.6 to 99%. Phylogenetic analysis of the B2L gene revealed that there are two main clusters of ORFV isolates which were responsible for past outbreaks in Turkey. The information presented here will provide an insight into genetic diversity of field isolates of ORFV circulating in the Central Anatolian region of Turkey.
羊口疮病毒（ORFV）是传染性脓疱性皮炎的病原体，可导致绵羊和山羊发生皮肤病。在本研究中，对土耳其安纳托利亚中部地区山羊的两起ORFV感染疫情进行了调查。2016年3月和5月期间，从阿克萨赖省和科尼亚省两个不同羊群中1至4月龄的羔羊（n = 9）采集样本。使用针对包膜基因（B2L）的特异性引物，通过PCR确认疑似样本中存在ORFV。B2L基因序列分析表明，本研究中两个分离株之间的核苷酸同源性为100%，而与来自不同地区的副痘病毒分离株的相似性范围为83.6%至99%。B2L基因的系统发育分析表明，有两个主要的ORFV分离株簇，它们是土耳其过去疫情的原因。此处提供的信息将有助于深入了解在土耳其安纳托利亚中部地区传播的ORFV野外分离株的遗传多样性。
