Yıldırım Yakup, Bilge Dağalp Seval, Bozkurt Gökhan, Doğan Fırat, Küçük Ali
Department of Virology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Türkiye.
Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Türkiye.
Vet Med Sci. 2025 Jan;11(1):e70139. doi: 10.1002/vms3.70139.
The orf virus (ORFV) is a viral pathogen that primarily causes contagious ecthyma in humans and different ruminants. The infection, which is common worldwide, causes large-scale economic losses to animal breeders.
In this study, tissue samples collected from eight randomly selected goats with dermatological lesions on the teats were examined in different goat herds. B2L gene-specific primer pairs (PP1, PP3 and PP4) were used to reveal the presence of ORFV by molecular methods and for phylogenetic analysis.
Viral DNA was detected in four of eight tissues using the semi-nested PCR method. In addition, the data obtained by performing sequence analyses of the amplicons with positive results were compared with the information of different ORFV isolates registered in the GenBank database. Based on the sequence analysis of the field isolates obtained in our study, it was found that the nucleotide similarities among these isolates and those from Asian countries were 100%. Furthermore, ORFV isolates collected from different species and produced in Türkiye over various periods exhibited homologous nucleotide sequences with similarities ranging from 98.1% to 98.8%. In the phylogenetic tree drawn based on the B2L genomic region, it was observed that our field isolates were classified in Group I together with other Turkish and Asian strains.
As a result, while other pathogenic agents are considered the cause of disease in goats with dermatological lesions on their mammary tissue, the ORFV should also be evaluated, and protection and control programs should be prepared accordingly.
羊口疮病毒（ORFV）是一种病毒病原体，主要引起人类和不同反刍动物的传染性脓疱性皮炎。这种感染在全球范围内普遍存在，给动物饲养者造成了大规模经济损失。
在本研究中，从随机挑选的八只乳头有皮肤病变的山羊身上采集组织样本，在不同的山羊群中进行检查。使用B2L基因特异性引物对（PP1、PP3和PP4），通过分子方法检测ORFV的存在并进行系统发育分析。
使用半巢式PCR方法在八个组织中的四个中检测到病毒DNA。此外，将扩增产物序列分析得到的阳性结果数据与GenBank数据库中登记的不同ORFV分离株信息进行比较。基于我们研究中获得的现场分离株的序列分析，发现这些分离株与来自亚洲国家的分离株之间的核苷酸相似性为100%。此外，在土耳其不同时期从不同物种中收集的ORFV分离株表现出同源核苷酸序列，相似性范围为98.1%至98.8%。在基于B2L基因组区域绘制的系统发育树中，观察到我们的现场分离株与其他土耳其和亚洲菌株一起被归类为第一组。
因此，当其他病原体被认为是乳腺组织有皮肤病变的山羊疾病的病因时，也应评估ORFV，并相应地制定保护和控制计划。
