2008 - 2013年埃塞俄比亚绵羊和山羊口疮病毒的分子特征
Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008-2013.
作者信息
Gelaye Esayas, Achenbach Jenna Elizabeth, Jenberie Shiferaw, Ayelet Gelagay, Belay Alebachew, Yami Martha, Loitsch Angelika, Grabherr Reingard, Diallo Adama, Lamien Charles Euloge
机构信息
Animal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400, Vienna, Austria.
Institute of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190, Vienna, Austria.
出版信息
Virol J. 2016 Feb 29;13:34. doi: 10.1186/s12985-016-0489-3.
BACKGROUND
Orf is a contagious disease of sheep, goats and wild ungulates caused by orf virus (ORFV) a member of the genus Parapoxvirus, Poxviridae family. Although orf is endemic in Ethiopia, little attention has been given so far as it is not a notifiable disease by the World Organization for Animal Health. In this work, we have investigated orf outbreaks representing five different geographical locations of Ethiopia, in Amba Giorgis, Gondar zuria, Adet, Debre zeit and Adami Tulu, between 2008 and 2013.
RESULTS
The viral isolation and the sequence analysis of the A32L and the B2L genes of eighteen representative isolates confirmed that sampled animals were infected by ORFVs. The phylogenetic study and the comparative analysis of the deduced amino acid profile suggests that there were two main clusters of ORFV isolates which were responsible for the investigated outbreaks. Additionally the analysis of these two genes showed limited variability to ORFVs encountered elsewhere. This is the first report on the genetic characterization of the ORFV isolates from sheep and goats in Ethiopia.
CONCLUSION
The molecular characterization of Ethiopian ORFV isolates highlighted the circulation of two main clusters causing orf disease in sheep and goats. The use of laboratory based methods and a constant monitoring of Ethiopian ORFV isolates is needed to better understand the dynamic of ORFV circulating in the country and facilitate the implementation of control measures.
背景
羊痘是由痘病毒科副痘病毒属的羊痘病毒（ORFV）引起的绵羊、山羊和野生有蹄类动物的一种传染病。尽管羊痘在埃塞俄比亚是地方病，但由于它不是世界动物卫生组织规定的须通报疾病，迄今很少受到关注。在这项工作中，我们调查了2008年至2013年间埃塞俄比亚五个不同地理位置（安巴乔吉斯、贡德尔祖里亚、阿代特、德布雷齐特和阿达米图卢）的羊痘疫情。
结果
对18株代表性分离株的A32L和B2L基因进行病毒分离和序列分析，证实采样动物感染了ORFV。系统发育研究和推导氨基酸谱的比较分析表明，有两个主要的ORFV分离株簇导致了所调查的疫情。此外，对这两个基因的分析显示，与其他地方发现的ORFV相比，其变异性有限。这是关于埃塞俄比亚绵羊和山羊中ORFV分离株基因特征的首次报告。
结论
埃塞俄比亚ORFV分离株的分子特征突出了导致绵羊和山羊羊痘疾病的两个主要簇的传播情况。需要使用基于实验室的方法并持续监测埃塞俄比亚的ORFV分离株，以更好地了解该国ORFV的传播动态，并促进控制措施的实施。
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