Giampieri Francesca, Gasparrini Massimiliano, Forbes-Hernandez Tamara Y, Mazzoni Luca, Capocasa Franco, Sabbadini Silvia, Alvarez-Suarez Josè M, Afrin Sadia, Rosati Carlo, Pandolfini Tiziana, Molesini Barbara, Sánchez-Sevilla José F, Amaya Iraida, Mezzetti Bruno, Battino Maurizio
Área de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI) , Campeche C.P.24040, México.
Grupo de Investigación en Biotecnologı́a Aplicada a Biomedicina (BIOMED), Facultad de Ciencias de la Salud, Universidad de Las Américas , Campus Queri - Bloque 7 - Planta Baja, EC170125 Quito, Ecuador.
J Agric Food Chem. 2018 Jan 24;66(3):581-592. doi: 10.1021/acs.jafc.7b04177. Epub 2018 Jan 8.
Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.
通过增加和/或调节生物活性化合物来强化食品已成为预防包括癌症在内的多种疾病的主要目标。在此，我们培育了用含有花青素合酶（ANS）基因的构建体转化的卡利普索草莓品系，以研究其对花青素生物合成、代谢和转录组的影响。对三个草莓ANS转基因品系（ANS L5、ANS L15和ANS L18）进行了植物化学成分和总抗氧化能力（TAC）分析，并评估了它们的果实提取物对肝癌细胞的细胞毒性作用。ANS L18果实的总酚类和黄酮类含量最高，而ANS L15的花青素浓度最高；TAC与总多酚含量呈正相关。果实转录组在多酚生物合成和其他相关代谢途径中也受到特异性影响。所有品系的果实提取物均以剂量/时间依赖性方式发挥细胞毒性作用，增加细胞凋亡和自由基水平，并损害线粒体功能。
