Bankoglu Ezgi Eyluel, Seyfried Florian, Arnold Charlotte, Soliman Alexander, Jurowich Christian, Germer Christoph Thomas, Otto Christoph, Stopper Helga
Institute of Pharmacology and Toxicology, University of Wuerzburg, Wuerzburg, Germany.
Department of General, Visceral, Vascular and Paediatric Surgery, University Hospital of Wuerzburg, Wuerzburg, Germany.
Mutagenesis. 2018 Feb 24;33(1):61-67. doi: 10.1093/mutage/gex040.
Obesity is associated with several detrimental health consequences, among them an increased risk for development of cancer, and an overall elevated mortality. Multiple factors like hyperinsulinemia, chronic microinflammation and oxidative stress may be involved. The comet assay has been proven to be very sensitive for detection of DNA damage and has been used to explore the relationship between overweight/obesity and DNA damage, but results are controversial. Very few investigations have been performed to correlate weight loss of obese individuals and possible reduction of DNA damage and these studies have not provided clear results. As currently, only surgical interventions (metabolic/bariatric surgery) enable substantial and sustained weight loss in the vast majority of morbidly obese patients, we analyzed whole blood samples of 56 subsequent patients prior, 6 and 12 months after bariatric surgery. No reduction of DNA damage was observed in comet assay analysis after 6 months despite efficient weight loss, but a significant reduction was observed 12 months after surgery. Concurrently, the ferric-reducing antioxidant power assay showed a significant reduction after 6 and 12 months. The level of oxidised glutathione and lipid peroxidation products were increased at 6 months but normalised at 12 months after surgery. As conclusion, a significant weight reduction in obese patients may help to diminish existing DNA damage besides improving many other health aspects in these patients.
肥胖与多种有害的健康后果相关，其中包括患癌风险增加以及总体死亡率升高。可能涉及多种因素，如高胰岛素血症、慢性微炎症和氧化应激。彗星试验已被证明对检测DNA损伤非常敏感，并已用于探索超重/肥胖与DNA损伤之间的关系，但结果存在争议。很少有研究将肥胖个体的体重减轻与DNA损伤可能的减少相关联，并且这些研究并未提供明确的结果。由于目前只有手术干预（代谢/减重手术）能使绝大多数病态肥胖患者实现显著且持续的体重减轻，我们分析了56例连续患者在减重手术前、术后6个月和12个月的全血样本。尽管体重有效减轻，但在术后6个月的彗星试验分析中未观察到DNA损伤减少，而在术后12个月观察到显著减少。同时，铁还原抗氧化能力试验显示在术后6个月和12个月有显著降低。氧化型谷胱甘肽和脂质过氧化产物水平在术后6个月升高，但在术后12个月恢复正常。结论是，肥胖患者显著减重除了能改善这些患者的许多其他健康方面外，还可能有助于减少现有的DNA损伤。
