Supekar Omkar D, Ozbay Baris N, Zohrabi Mo, Nystrom Philip D, Futia Gregory L, Restrepo Diego, Gibson Emily A, Gopinath Juliet T, Bright Victor M
Department of Mechanical Engineering, University of Colorado, Boulder, CO, 80309, USA.
Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045, USA.
Biomed Opt Express. 2017 Nov 7;8(12):5412-5426. doi: 10.1364/BOE.8.005412. eCollection 2017 Dec 1.
Laser scanners are an integral part of high resolution biomedical imaging systems such as confocal or 2-photon excitation (2PE) microscopes. In this work, we demonstrate the utility of electrowetting on dielectric (EWOD) prisms as a lateral laser-scanning element integrated in a conventional 2PE microscope. To the best of our knowledge, this is the first such demonstration for EWOD prisms. EWOD devices provide a transmissive, low power consuming, and compact alternative to conventional adaptive optics, and hence this technology has tremendous potential. We demonstrate 2PE microscope imaging of cultured mouse hippocampal neurons with a FOV of 130 × 130 μm using EWOD prism scanning. In addition, we show simulations of the optical system with the EWOD prism, to evaluate the effect of propagating a Gaussian beam through the EWOD prism on the imaging quality. Based on the simulation results a beam size of 0.91 mm full width half max was chosen to conduct the imaging experiments, resulting in a numerical aperture of 0.17 of the imaging system.
激光扫描仪是高分辨率生物医学成像系统（如共聚焦或双光子激发（2PE）显微镜）不可或缺的一部分。在这项工作中，我们展示了介电层上电润湿（EWOD）棱镜作为集成在传统2PE显微镜中的横向激光扫描元件的实用性。据我们所知，这是首次对EWOD棱镜进行此类演示。EWOD器件为传统自适应光学提供了一种透射式、低功耗且紧凑的替代方案，因此这项技术具有巨大潜力。我们使用EWOD棱镜扫描展示了对培养的小鼠海马神经元进行的2PE显微镜成像，视野为130×130μm。此外，我们展示了带有EWOD棱镜的光学系统的模拟，以评估高斯光束通过EWOD棱镜传播对成像质量的影响。基于模拟结果，选择半高全宽为0.91mm的光束尺寸进行成像实验，从而使成像系统的数值孔径达到0.17。
