Bocarsly Miriam E, Jiang Wan-Chen, Wang Chen, Dudman Joshua T, Ji Na, Aponte Yeka
Intramural Research Program, Neuronal Circuits and Behavior Unit, National Institute on Drug Abuse, Baltimore, MD 21224, USA ; Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA ; These authors contributed equally to this work ; Current address: Section on Neuronal Structure, National Institute on Alcohol Abuse and Alcoholism, National Institute of Health, Bethesda, MD 20892, USA.
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA ; These authors contributed equally to this work.
Biomed Opt Express. 2015 Oct 23;6(11):4546-56. doi: 10.1364/BOE.6.004546. eCollection 2015 Nov 1.
The ability to image neurons anywhere in the mammalian brain is a major goal of optical microscopy. Here we describe a minimally invasive microendoscopy system for studying the morphology and function of neurons at depth. Utilizing a guide cannula with an ultrathin wall, we demonstrated in vivo two-photon fluorescence imaging of deeply buried nuclei such as the striatum (2.5 mm depth), substantia nigra (4.4 mm depth) and lateral hypothalamus (5.0 mm depth) in mouse brain. We reported, for the first time, the observation of neuronal activity with subcellular resolution in the lateral hypothalamus and substantia nigra of head-fixed awake mice.
对哺乳动物大脑中任何位置的神经元进行成像的能力是光学显微镜的一个主要目标。在此，我们描述了一种用于深入研究神经元形态和功能的微创显微内窥镜系统。利用具有超薄壁的引导套管，我们在小鼠大脑中对诸如纹状体（深度2.5毫米）、黑质（深度4.4毫米）和外侧下丘脑（深度5.0毫米）等深埋核团进行了体内双光子荧光成像。我们首次报告了在头部固定的清醒小鼠的外侧下丘脑和黑质中以亚细胞分辨率观察神经元活动。
