Institute of Marine Drugs, School of Pharmaceutical Sciences, Guangxi University of Chinese Medicine, No. 13 Wuhe Avenue, Nanning, 530001, People's Republic of China.
Arch Microbiol. 2022 Feb 15;204(3):174. doi: 10.1007/s00203-021-02674-z.
A bacterial isolate (BGMRC 2046) was isolated from the rhizosphere soil of Zoysia matrella collected from the Beibu Gulf of China. The results of a polyphasic taxonomic study revealed that this strain belongs to a member of the genus Stappia with the characteristics of Gram-stain-negative, catalase- and oxidase-positive, motile, short rod-shaped. The strain grew at 20-37 °C (optimal, 28 °C), pH 6.0-9.0 (optimal, pH 7.0), and 1-7% (w/v) optimal, NaCl (1-3%). A phylogenetic evaluation based on 16S rRNA gene sequence analysis revealed that this strain fall into the family Stappiaceae, being most closely related to Stappia indica CGMCC 1A01226 (95.8% sequence similarity), Stappia stellulata DSM 5886 (95.1%), and Stappia taiwanensis DSM 23284 (94.4%). The major cellular fatty acid, respiratory quinone and polar lipids were all detected from new species (BGMRC 2046T), that shows the chemical characteristics of BGMRC 2046T. The major polar lipids were two unidentified ninhydrin positive phospholipids, four unidentified phospholipids, and one unidentified lipid. Genome sequencing revealed a genome size of 4.78 Mbp and a G + C content of 60.8%. Pairwise comparison of the genomes of the new strain BGMRC 2046 and the three most closely related strains resulted in gANI values was lower than 75% and a digital DNA-DNA hybridization values was lower than 24%. The strain possessed genes encoding choline uptake and conversion to betaine gene clusters. The results of the polyphasic taxonomic study showed that strain BGMRC 2046 represents a new species of the genus Stappia. The name Stappia sediminis sp. nov. is proposed for the species with the type strain BGMRC 2046 (= KCTC52115 = CGMCC1.17425).
从采自中国北部湾的中华结缕草根际土壤中分离到一株细菌分离物（BGMRC 2046）。多相分类学研究的结果表明，该菌株属于 Stappia 属的一个成员，具有革兰氏阴性、过氧化氢酶和氧化酶阳性、运动性、短杆状的特征。该菌株在 20-37°C（最佳，28°C）、pH 6.0-9.0（最佳，pH 7.0）和 1-7%（w/v）最佳、NaCl（1-3%）下生长。基于 16S rRNA 基因序列分析的系统发育评估表明，该菌株属于 Stappiaceae 科，与 Stappia indica CGMCC 1A01226（95.8%序列相似性）、Stappia stellulata DSM 5886（95.1%）和 Stappia taiwanensis DSM 23284（94.4%）最为密切相关。新种（BGMRC 2046T）中检测到主要细胞脂肪酸、呼吸醌和极性脂，表明 BGMRC 2046T 的化学特征。主要极性脂为两种未鉴定的茚三酮阳性磷脂、四种未鉴定的磷脂和一种未鉴定的脂。基因组测序揭示了一个 4.78 Mbp 的基因组大小和 60.8%的 G+C 含量。新菌株 BGMRC 2046 与三种最密切相关的菌株的基因组进行两两比较，结果表明 gANI 值低于 75%，数字 DNA-DNA 杂交值低于 24%。该菌株具有编码胆碱摄取和转化为甜菜碱基因簇的基因。多相分类学研究的结果表明，菌株 BGMRC 2046 代表 Stappia 属的一个新种。建议将该种命名为 Sediminis sp. nov.，其模式菌株为 BGMRC 2046（=KCTC52115=CGMCC1.17425）。
