Vector and Waterborne Pathogens Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia.
Parasit Vectors. 2018 Jan 4;11(1):12. doi: 10.1186/s13071-017-2550-5.
The tick microbiome comprises communities of microorganisms, including viruses, bacteria and eukaryotes, and is being elucidated through modern molecular techniques. The advent of next-generation sequencing (NGS) technologies has enabled the genes and genomes within these microbial communities to be explored in a rapid and cost-effective manner. The advantages of using NGS to investigate microbiomes surpass the traditional non-molecular methods that are limited in their sensitivity, and conventional molecular approaches that are limited in their scalability. In recent years the number of studies using NGS to investigate the microbial diversity and composition of ticks has expanded. Here, we provide a review of NGS strategies for tick microbiome studies and discuss the recent findings from tick NGS investigations, including the bacterial diversity and composition, influential factors, and implications of the tick microbiome.
蜱虫微生物组由微生物群落组成，包括病毒、细菌和真核生物，并且正在通过现代分子技术进行阐明。下一代测序（NGS）技术的出现使人们能够以快速且具有成本效益的方式探索这些微生物群落中的基因和基因组。使用 NGS 来研究微生物组的优势超过了传统的非分子方法，这些方法在灵敏度上受到限制，而传统的分子方法在可扩展性上受到限制。近年来，使用 NGS 来研究蜱虫微生物多样性和组成的研究数量有所增加。在这里，我们提供了蜱虫微生物组研究的 NGS 策略综述，并讨论了蜱虫 NGS 研究的最新发现，包括细菌多样性和组成、影响因素以及蜱虫微生物组的意义。
