用于从土壤和根际环境中分离嗜麦芽窄食单胞菌的选择性培养基。
Selective medium for isolation of Xanthomonas maltophilia from soil and rhizosphere environments.
作者信息
Juhnke M E, des Jardin E
机构信息
Fort Lauderdale Research and Education Center, University of Florida 33314.
出版信息
Appl Environ Microbiol. 1989 Mar;55(3):747-50. doi: 10.1128/aem.55.3.747-750.1989.
Abstract
A selective medium (XMSM) was developed for isolation of Xanthomonas maltophilia from bulk soil and plant rhizosphere environments. The XMSM basal medium contained maltose, tryptone, bromthymol blue, and agar. Antibiotics added to select for X. maltophilia were cycloheximide, nystatin, cephalexin, bacitracin, penicillin G, novobiocin, neomycin sulfate, and tobramycin. A comparison was made between XMSM and 1/10-strength tryptic soy broth agar for recovery of X. maltophilia from sterile and nonsterile soil infested with known X. maltophilia isolates. A recovery rate of 97% or greater for XMSM was demonstrated. XMSM was used to isolate X. maltophilia from a variety of soil and rhizosphere environments.
摘要
开发了一种选择性培养基（XMSM），用于从大量土壤和植物根际环境中分离嗜麦芽窄食单胞菌。XMSM基础培养基含有麦芽糖、胰蛋白胨、溴百里酚蓝和琼脂。添加用于选择嗜麦芽窄食单胞菌的抗生素有放线菌酮、制霉菌素、头孢氨苄、杆菌肽、青霉素G、新生霉素、硫酸新霉素和妥布霉素。对XMSM和1/10强度胰蛋白胨大豆肉汤琼脂从接种已知嗜麦芽窄食单胞菌分离株的无菌和非无菌土壤中回收嗜麦芽窄食单胞菌的情况进行了比较。结果表明XMSM的回收率达到97%或更高。XMSM被用于从各种土壤和根际环境中分离嗜麦芽窄食单胞菌。
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