Yao J D, Louie M, Louie L, Goodfellow J, Simor A E
Department of Microbiology, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.
J Clin Microbiol. 1995 May;33(5):1428-30. doi: 10.1128/jcm.33.5.1428-1430.1995.
Currently recommended dilution test methods for the determination of antimicrobial susceptibility of Stenotrophomonas (Xanthomonas) maltophilia are labor-intensive and often impractical in many clinical laboratories. We compared the E test with the agar dilution method for susceptibility testing of 176 clinical isolates of S. maltophilia against 16 antimicrobial agents. The MICs obtained by E test correlated well with those determined by the agar dilution method, with an overall agreement of 94%. Very major and major errors occurred infrequently (0.6 to 2.9%) when testing beta-lactam agents, tobramycin, trimethoprim-sulfamethoxazole, and fluoroquinolones. The E test was found to be accurate and easy to perform. For most antimicrobial agents tested against S. maltophilia, the E test is an acceptable alternative susceptibility test method.
目前推荐的用于嗜麦芽窄食单胞菌(黄单胞菌属)药敏测定的稀释试验方法劳动强度大,在许多临床实验室中往往不实用。我们将E试验与琼脂稀释法进行比较,对176株嗜麦芽窄食单胞菌临床分离株针对16种抗菌药物进行药敏试验。E试验获得的最低抑菌浓度(MIC)与琼脂稀释法测定的结果相关性良好,总体一致性为94%。在检测β-内酰胺类药物、妥布霉素、甲氧苄啶-磺胺甲恶唑和氟喹诺酮类药物时,极重大和重大错误很少发生(0.6%至2.9%)。发现E试验准确且易于操作。对于大多数针对嗜麦芽窄食单胞菌检测的抗菌药物,E试验是一种可接受的替代药敏试验方法。