Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China.
Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China; National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.
Plant Physiol Biochem. 2018 Feb;123:378-391. doi: 10.1016/j.plaphy.2017.12.034. Epub 2017 Dec 26.
Plant homeodomain (PHD)-finger proteins are a class of important zinc-finger transcription factors responsible for regulating transcription and the chromatin state and responsive to various stresses. The family genes have been reported in many plants, but there is little information about PHD-finger genes in moso bamboo. In this study, 60 PHD-finger genes (PePHD1-60) were identified in moso bamboo and classified into 11 subfamilies (A-K) based on phylogenetic analysis. Gene structure and conserved motif analysis showed that these genes contained different numbers of introns but had similar motif organizations within each subfamily. Multiple sequence alignment revealed that the PHD-finger proteins possessed conserved structural domain sequences. In addition, the family underwent purifying selection during evolution and experienced a large-scale duplication event around 7.69-15.4 million years ago. Most importantly, the expression profiles of young leaves (YL), mature leaves (L), roots (R), stems (S), shoots (Sh) and rhizomes (Rh) displayed that they might involve in the formation of these tissues. Based on promoter analysis of 16 putative stress-related genes, quantitative real-time PCR assays were performed using moso bamboo leaves and showed that these genes were differentially regulated under abscisic acid (ABA), drought, low temperature and NaCl treatments. Therefore, the results reveal that PePHD genes play crucial roles in organ formation and response to multiple environmental stress conditions of moso bamboo, which will make for further function analysis of PHD-finger genes in plants.
植物同源结构域（PHD）-指蛋白是一类重要的锌指转录因子，负责调节转录和染色质状态，并对各种应激作出响应。该家族基因已在许多植物中报道，但关于毛竹 PHD 指蛋白基因的信息较少。本研究在毛竹中鉴定出 60 个 PHD 指蛋白基因（PePHD1-60），并根据系统发育分析将其分为 11 个亚家族（A-K）。基因结构和保守基序分析表明，这些基因包含不同数量的内含子，但在每个亚家族内具有相似的基序组织。多序列比对显示，PHD 指蛋白具有保守的结构域序列。此外，该家族在进化过程中经历了纯化选择，并在 769 万至 1540 万年前发生了大规模的复制事件。最重要的是，幼叶（YL）、成熟叶（L）、根（R）、茎（S）、芽（Sh）和根茎（Rh）的表达谱显示，它们可能参与这些组织的形成。对 16 个假定与胁迫相关基因的启动子分析后，使用毛竹叶片进行了定量实时 PCR 检测，结果表明这些基因在脱落酸（ABA）、干旱、低温和 NaCl 处理下的表达水平存在差异。因此，研究结果表明 PePHD 基因在毛竹器官形成和对多种环境胁迫条件的响应中发挥着关键作用，这将有助于进一步分析 PHD 指蛋白基因在植物中的功能。
