Arciero D, Vannelli T, Logan M, Hooper A B
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Biochem Biophys Res Commun. 1989 Mar 15;159(2):640-3. doi: 10.1016/0006-291x(89)90042-9.
Suspensions of Nitrosomonas europaea are shown to cause the complete disappearance of 10 microM trichloroethylene at rates of 1 microM mg protein-1. The reaction continues at nearly this rate for many hours. Fresh cells catalyze the reaction in the absence of added ammonium (presumably utilizing endogenous ammonia or stored reductant). In older cells, trichloroethylene degradation depends on the addition of ammonia. Acetylene, 2-chloro 6-trichloromethylpyridine and alpha alpha'dipyridyl, which inhibit the oxidation of ammonia by cells, inhibit the degradation of trichloroethylene. Thus degradation of trichloroethylene is dependent on- and possibly catalyzed by the ammonia oxidizing enzyme.
欧洲亚硝化单胞菌的悬浮液被证明能以1微摩尔每毫克蛋白质的速率使10微摩尔的三氯乙烯完全消失。该反应能以几乎这个速率持续进行许多小时。新鲜细胞在不添加铵（推测利用内源性氨或储存的还原剂）的情况下催化反应。在较老的细胞中，三氯乙烯的降解依赖于氨的添加。抑制细胞对氨氧化的乙炔、2-氯-6-三氯甲基吡啶和α,α'-联吡啶，也抑制三氯乙烯的降解。因此，三氯乙烯的降解依赖于氨氧化酶，并且可能由其催化。
