Fallik Noam, Bar-Lavan Yael, Greenshpan Yariv, Goldstein Oron, Grosch Markus, Drukker Micha, Gazit Roi
The Shraga Segal Department for Microbiology Immunology and Genetics, Faculty of Health Sciences, The Ben-Gurion University of the Negev, Be'er Sheva, Israel.
National Institute for Biotechnology in the Negev, The Ben-Gurion University of the Negev, Be'er Sheva, Israel.
Oncotarget. 2017 Nov 30;8(65):109575-109586. doi: 10.18632/oncotarget.22729. eCollection 2017 Dec 12.
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs (lncRNA) in HSCs. is a lncRNA that plays a major role in the formation of sub-nuclear structures called paraspeckles, and was reported to regulate proliferation and differentiation in other cells types. We detected expression using RNA-seq data and RT-qPCR in HSCs, progenitors and effector immune cells, by specific detection of its isoforms. is highly expressed in stem and progenitor cells, yet it shows significant reduction in granulocytes. Microscopically, is detected as sharp nuclear foci. Paraspeckle proteins NONO and PSPC1 are detected as aggregated nuclear foci in fresh primary hematopoietic cells, and in cultured cells. Induction of differentiation was found to enhance expression. Taken together, our data demonstrate for the first time the expression of and paraspeckles formation in HSCs and along hematopoiesis.
造血干细胞（HSCs）通过分级分化过程产生血液和免疫细胞。调控这一过程的基因对于理解正常和恶性造血作用具有重要意义。然而，令人惊讶的是，人们对造血干细胞中的长链非编码RNA（lncRNA）知之甚少。是一种lncRNA，在称为旁斑的亚核结构形成中起主要作用，并且据报道在其他细胞类型中调节增殖和分化。我们通过RNA测序数据和RT-qPCR在造血干细胞、祖细胞和效应免疫细胞中检测的表达，通过特异性检测其异构体。在干细胞和祖细胞中高表达，但在粒细胞中显著降低。在显微镜下，被检测为清晰的核灶。在新鲜的原代造血细胞和培养细胞中，旁斑蛋白NONO和PSPC1被检测为聚集的核灶。发现诱导分化可增强的表达。综上所述，我们的数据首次证明了在造血干细胞中以及造血过程中的表达和旁斑形成。
