Department of Pediatric Dentistry and Public Health, Araçatuba Dental School, Universidade Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil.
Engeneering School, Universidade Estadual Paulista (UNESP), Ilha Solteira, São Paulo, Brazil.
J Biomed Mater Res B Appl Biomater. 2018 Oct;106(7):2553-2564. doi: 10.1002/jbm.b.34072. Epub 2018 Jan 4.
This study evaluated the effect of sodium trimetaphosphate (TMP) associated or not with fluoride (F) on the structure and dissolution of carbonated hydroxyapatite (CHA). Synthetic CHA powder (1.0 g) was suspended in solutions containing TMP at 0-10%, associated with 0, 1100, 4500, or 9000 ppm F, and the precipitates were submitted to a pH cycle (n = 6/group). Samples were analyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction. F, calcium (Ca) and phosphorous (P) concentrations were determined in CHA, while P and F were analyzed in the supernatants. Data were submitted to analysis of variance, Student-Newman-Keuls' test and Pearson's correlation coefficient (α = 0.05). Solutions containing 1100 ppm F with TMP between 2-4% and 4500 and 9000 ppm F with TMP between 4 and 8% led to higher Ca/P ratio (p < 0.05) in CHA. Alkali-soluble F deposition was directly related to TMP concentrations whereas an inverse pattern was observed for acid-soluble F incorporation (p < 0.05). Greater P adsorption in the CHA structure was observed with increasing concentrations of TMP for the 0 and 1100 ppm F solutions (p < 0.05). All diffractograms and FTIR spectra showed a similar pattern to that for pure hydroxyapatite. Thus, when TMP and F are coadministered, TMP interferes with F deposition on CHA, and an ideal TMP:F ratio can enhance the precipitation of CHA with lower solubility. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2553-2564, 2018.
这项研究评估了三聚磷酸钠（TMP）与氟化物（F）联合或不联合对碳酸羟磷灰石（CHA）结构和溶解的影响。将 1.0 g 合成 CHA 粉末悬浮在含有 0-10% TMP 的溶液中，同时含有 0、1100、4500 或 9000 ppm F，沉淀接受 pH 循环（每组 n = 6）。通过傅里叶变换红外光谱（FTIR）和 X 射线衍射分析样品。在 CHA 中测定 F、钙（Ca）和磷（P）浓度，在上清液中分析 P 和 F。数据采用方差分析、Student-Newman-Keuls 检验和 Pearson 相关系数（α=0.05）进行分析。含 1100 ppm F 的 TMP 浓度在 2-4%之间，4500 和 9000 ppm F 的 TMP 浓度在 4-8%之间的溶液导致 CHA 中 Ca/P 比值更高（p<0.05）。可溶 F 的沉积与 TMP 浓度直接相关，而酸溶性 F 的掺入则呈相反模式（p<0.05）。随着 TMP 浓度的增加，观察到 CHA 结构中 P 的吸附量增加，对于 0 和 1100 ppm F 溶液，P 的吸附量增加（p<0.05）。所有的衍射图谱和 FTIR 光谱均显示出与纯羟磷灰石相似的模式。因此，当 TMP 和 F 共同给药时，TMP 会干扰 F 在 CHA 上的沉积，并且理想的 TMP:F 比值可以增强 CHA 的沉淀，同时降低其溶解度。©2018 Wiley Periodicals, Inc. J 生物材料 Res 部分 B: 应用生物材料，106B：2553-2564，2018。
