Department of Pediatric Dentistry and Public Health, School of Dentistry, Araçatuba, São Paulo State University (UNESP), Araçatuba, SP, Brazil.
Department of Physics and Chemistry, São Paulo State University (UNESP), Ilha Solteira, SP, Brazil.
Clin Oral Investig. 2018 Dec;22(9):3021-3029. doi: 10.1007/s00784-018-2384-3. Epub 2018 Feb 20.
This in vitro study evaluated the effect of microparticles (TMPmicro) or nanoparticles (TMPnano) TMP associated with fluoride (F) in toothpaste formulations on the obliteration of dentinal tubules (DT).
The dentinal tubules of bovine dentin blocks were unobstructed by immersion in 37% phosphoric acid solution. Blocks (n = 20/group) underwent mechanical brushing (2×/day) during 7 days with toothpastes: placebo, 1100 ppm F, and 1100 with 3% TMPmicro or 3% TMPnano. After that, ten blocks of each group were immersed in citric acid (pH 3.2) for 1 min. The number, diameter and area of unobstructed DT, atomic % of chemical elements from precipitates, and the mineral concentration were quantified. Data were submitted to two-way ANOVA, followed by Student-Newman-Keuls test (P < 0.05).
Toothpastes containing TMPmicro and TMPnano promoted greater obliteration of DT and greater mineral concentration compared to other groups (P < 0.05). Placebo and 1100 ppm F group presented similar obliteration but 1100 ppm F group promoted higher mineral concentration. Higher Ca/P ratios were observed in groups treated with TMP; the acid challenge reduced Ca/P ratio for all groups. The atomic % of C and N was significantly lower for TMP toothpastes before acid challenge, but increased afterwards.
It was concluded that the addition of TMPmicro or TMPnano to F toothpastes produced greater obliteration of dentinal tubules and higher mineral concentration when compared with 1100 ppm F.
By the addition of TMP, fluoride toothpastes may occlude the dentinal tubules, with potential to reduce dentin hypersensitivity.
本体外研究评估了含氟（F）的微粒（TMPmicro）或纳米粒（TMPnano）TMP 与牙膏配方中 TMP 联合对牙本质小管（DT）闭塞的影响。
将牛牙本质块的牙本质小管用 37%磷酸溶液浸泡使其未阻塞。将（n=20/组）块在 7 天内每天两次机械刷牙，使用牙膏：安慰剂、1100ppm F 和 1100ppm F 加 3% TMPmicro 或 3% TMPnano。之后，每组的 10 个块被浸入柠檬酸（pH 3.2）中 1 分钟。对未阻塞的 DT 的数量、直径和面积、沉淀物的化学元素的原子%和矿物浓度进行定量。数据进行双因素方差分析，然后进行 Student-Newman-Keuls 检验（P<0.05）。
与其他组相比，含 TMPmicro 和 TMPnano 的牙膏促进了 DT 的更大闭塞和更高的矿物浓度（P<0.05）。安慰剂和 1100ppm F 组表现出相似的闭塞，但 1100ppm F 组促进了更高的矿物浓度。用 TMP 处理的组观察到更高的 Ca/P 比值；所有组的酸挑战都降低了 Ca/P 比值。在酸挑战之前，用 TMP 处理的牙膏的 C 和 N 的原子%显著较低，但之后增加。
结论是，与 1100ppm F 相比，在 F 牙膏中添加 TMPmicro 或 TMPnano 可产生更大的牙本质小管闭塞和更高的矿物浓度。
通过添加 TMP，氟化物牙膏可能会闭塞牙本质小管，从而有潜力降低牙本质敏感。
