Zahiruddin Sultan, Parveen Abida, Khan Washim, Ibrahim Mohammad, Want Muzamil Y, Parveen Rabea, Ahmad Sayeed
Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
Department of Clinical Research, School of Interdisciplinary Sciences and Technology, Jamia Hamdard, New Delhi, India.
Front Pharmacol. 2022 Jan 3;12:647244. doi: 10.3389/fphar.2021.647244. eCollection 2021.
The study was aimed to develop a characterized polyherbal combination as an immunomodulator containing L., L., (L.) Dunal, and (Willd.) Miers. Through response surface methodology (RSM), the ratio of aqueous extracts of four plant materials was optimized and comprised 49.76% of , 1.35% of , 5.41% of , and 43.43% of for optimum immunomodulatory activity. The optimized combination showed antioxidant potential and contains more than 180 metabolites, out of which gallic acid, quercetin, ellagic acid, caffeic acid, kaempferitrin, and -coumaric acid are some common and significant metabolites found in plant extracts and in polyherbal combination. Treatment with the polyherbal combination of different doses in cyclophosphamide-induced immunosuppressed mice significantly (p < 0.01) enhanced the subsets of immune cells such as natural killer (NK) cells (60%), B cells (18%), CD4 cells (14%), and CD8 cells (7%). The characterized polyherbal combination exhibited potent immunomodulatory activity, which can be further explored clinically for its therapeutic applicability.
本研究旨在开发一种具有特征的多草药组合作为免疫调节剂，其包含[植物名称1]、[植物名称2]、[植物名称3]和[植物名称4]。通过响应面法（RSM），优化了四种植物材料水提取物的比例，为获得最佳免疫调节活性，其组成为[植物名称1]占49.76%、[植物名称2]占1.35%、[植物名称3]占5.41%、[植物名称4]占43.43%。优化后的组合具有抗氧化潜力，含有180多种代谢物，其中没食子酸、槲皮素、鞣花酸、咖啡酸、山柰酚苷和对香豆酸是在植物提取物和多草药组合中发现的一些常见且重要的代谢物。用不同剂量的多草药组合处理环磷酰胺诱导的免疫抑制小鼠，显著（p < 0.01）增加了免疫细胞亚群，如自然杀伤（NK）细胞（增加60%）、B细胞（增加18%）、CD4细胞（增加14%）和CD8细胞（增加7%）。这种具有特征的多草药组合表现出强大的免疫调节活性，其治疗适用性可在临床上进一步探索。
