Xu Zhongxian, Jie Hang, Chen Binlong, Gaur Uma, Wu Nan, Gao Jian, Li Pinming, Zhao Guijun, Zeng Dejun, Yang Mingyao, Li Diyan
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
J Genet. 2017 Dec;96(6):1033-1040. doi: 10.1007/s12041-017-0872-x.
The Chinese forest musk deer (Moschus berezovskii Flerov) is an endangered artiodactyl mammal. The musk secreted by sexually mature males is highly valued for alleged pharmaceutical properties and perfume manufacturing. However, the genomic and transcriptomic resources of musk deer remain deficiently represented and poorly understood. Next-generation sequencing technique is an efficient method for generating an enormous amount of sequence data that can represent a large number of genes and their expression levels. In the present study, we used Illumina HiSeq technology to perform de novo assembly of heart and musk gland transcriptomes from the Chinese forest musk deer. A total of 239,383 transcripts and 176,450 unigenes were obtained, of which 37,329 unigenes were matched to known sequences in the NCBI nonredundant protein (Nr) database; 31,039 unigenes were assigned to 61 GO terms, and 11,782 to 332 KEGG pathways. Additionally, 592 and 2282 differentially expressed genes were found to be specifically expressed in the heart and musk gland, respectively. The abundant transcriptomic data generated in the present report will provide a comprehensive sequence resource for Chinese forest musk deer as well as lay down a foundation which will help in accelerating genetic and functional genomics research in this species.
中国林麝（Moschus berezovskii Flerov）是一种濒危的偶蹄目哺乳动物。性成熟雄性林麝分泌的麝香因其所谓的药用特性和香水制造价值而备受珍视。然而，麝的基因组和转录组资源仍然缺乏且了解甚少。新一代测序技术是一种高效的方法，可生成大量能代表众多基因及其表达水平的序列数据。在本研究中，我们使用Illumina HiSeq技术对中国林麝的心脏和麝香腺转录组进行了从头组装。共获得了239,383个转录本和176,450个单基因，其中37,329个单基因与NCBI非冗余蛋白质（Nr）数据库中的已知序列匹配；31,039个单基因被归类到61个基因本体（GO）术语中，11,782个单基因被归类到332条京都基因与基因组百科全书（KEGG）通路中。此外，分别发现592个和2282个差异表达基因在心脏和麝香腺中特异性表达。本报告中生成的丰富转录组数据将为中国林麝提供全面的序列资源，并为加速该物种的遗传和功能基因组学研究奠定基础。
