Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation,Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing, 100083, China.
Shaanxi Institute of Zoology, No. 88 Xing Qing Ave Xian, Shaanxi, 710032, China.
Sci Rep. 2018 Jan 12;8(1):599. doi: 10.1038/s41598-017-18534-0.
Forest musk deer (Moschus berezovskii; FMD) are both economically valuable and highly endangered. A problem for FMD captive breeding programs has been the susceptibility of FMD to abscesses. To investigate the mechanisms of abscess development in FMD, the blood transcriptomes of three purulent and three healthy individuals were generated. A total of ~39.68 Gb bases were generated using Illumina HiSeq 4000 sequencing technology and 77,752 unigenes were identified after assembling. All the unigenes were annotated, with 63,531 (81.71%) mapping to at least one database. Based on these functional annotations, 45,798 coding sequences (CDS) were detected, along with 12,697 simple sequence repeats (SSRs) and 65,536 single nucleotide polymorphisms (SNPs). A total of 113 unigenes were found to be differentially expressed between healthy and purulent individuals. Functional annotation indicated that most of these differentially expressed genes were involved in the regulation of immune system processes, particularly those associated with parasitic and bacterial infection pathways.
林麝（Moschus berezovskii；FMD）兼具经济价值和高度濒危性。林麝圈养繁殖计划的一个问题是，林麝易患脓肿。为了研究林麝脓肿发展的机制，我们生成了三例化脓和三例健康个体的血液转录组。使用 Illumina HiSeq 4000 测序技术共生成了约 39.68Gb 碱基，组装后鉴定出 77752 个基因。所有基因都进行了注释，其中 63531 个（81.71%）至少注释到一个数据库。基于这些功能注释，共检测到 45798 个编码序列（CDS），12697 个简单重复序列（SSR）和 65536 个单核苷酸多态性（SNP）。在健康个体和化脓个体之间发现了 113 个差异表达的基因。功能注释表明，这些差异表达基因中的大多数参与了免疫系统过程的调节，特别是与寄生虫和细菌感染途径相关的基因。
