Tang Jie, Suo Lijuan, Li Feiran, Yang Chao, Bian Kun, Wang Yan
Shaanxi Key Laboratory for Animal Conservation, Shaanxi Institute of Zoology, Xi'an, China.
Front Vet Sci. 2022 Oct 26;9:1012276. doi: 10.3389/fvets.2022.1012276. eCollection 2022.
Pneumonia can seriously threaten the life of forest musk deer (FMD, an endangered species). To gain a comprehensive understanding of pneumonia pathogenesis in FMD, iTRAQ-based proteomics analysis was performed in diseased (Pne group) lung tissues of FMD that died of pneumonia and normal lung tissues (Ctrl group) of FMD that died from fighting against each other. Results showed that 355 proteins were differentially expressed (fold change ≥ 1.2 and adjusted -value < 0.05) in Pne vs. Ctrl. GO/KEGG annotation and enrichment analyses showed that dysregulated proteins might play vital roles in bacterial infection and immunity. Given the close association between bacterial infection and pneumonia, 32 dysregulated proteins related to infection, bacterial invasion of epithelial cells, and pathogenic infection were screened out. Among these 32 proteins, 13 proteins were mapped to the bovine genome. Given the close phylogenetic relationships of FMD and bovine, the protein-protein interaction networks of the above-mentioned 13 proteins were constructed by the String database. Based on the node degree analysis, 5 potential key proteins related to pneumonia-related bacterial infection in FMD were filtered out. Moreover, 85 dysregulated proteins related to the immune system process were identified given the tight connection between immune dysregulation and pneumonia pathogenesis. Additionally, 12 proteins that might function as crucial players in pneumonia-related immune response in FMD were screened out using the same experimental strategies described above. In conclusion, some vital proteins, biological processes, and pathways in pneumonia development were identified in FMD.
肺炎会严重威胁林麝（一种濒危物种）的生命。为全面了解林麝肺炎的发病机制，对死于肺炎的林麝患病肺组织（肺炎组）和死于相互打斗的林麝正常肺组织（对照组）进行了基于iTRAQ的蛋白质组学分析。结果显示，肺炎组与对照组相比，有355种蛋白质差异表达（倍数变化≥1.2且校正P值<0.05）。基因本体论/京都基因与基因组百科全书注释及富集分析表明，失调的蛋白质可能在细菌感染和免疫中发挥重要作用。鉴于细菌感染与肺炎密切相关，筛选出了32种与感染、细菌侵袭上皮细胞和病原体感染相关的失调蛋白质。在这32种蛋白质中，有13种蛋白质可映射到牛基因组。鉴于林麝与牛的系统发育关系密切，通过String数据库构建了上述13种蛋白质的蛋白质-蛋白质相互作用网络。基于节点度分析，筛选出了5种与林麝肺炎相关细菌感染潜在的关键蛋白质。此外，鉴于免疫失调与肺炎发病机制紧密相关，鉴定出了85种与免疫系统过程相关的失调蛋白质。另外，使用上述相同实验策略筛选出了12种可能在林麝肺炎相关免疫反应中起关键作用的蛋白质。总之，在林麝中鉴定出了肺炎发展过程中的一些重要蛋白质、生物学过程和信号通路。
