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三磷酸腺苷修饰小胶质细胞释放的细胞外囊泡蛋白质组并影响其对星形胶质细胞的作用。

ATP Modifies the Proteome of Extracellular Vesicles Released by Microglia and Influences Their Action on Astrocytes.

作者信息

Drago Francesco, Lombardi Marta, Prada Ilaria, Gabrielli Martina, Joshi Pooja, Cojoc Dan, Franck Julien, Fournier Isabelle, Vizioli Jacopo, Verderio Claudia

机构信息

Univ. Lille, INSERM, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM, Lille, France.

Fondazione Istituto Oncologico del Mediterraneo, Viagrande, Italy.

出版信息

Front Pharmacol. 2017 Dec 13;8:910. doi: 10.3389/fphar.2017.00910. eCollection 2017.

DOI:10.3389/fphar.2017.00910
PMID:29321741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5733563/
Abstract

Extracellular ATP is among molecules promoting microglia activation and inducing the release of extracellular vesicles (EVs), which are potent mediators of intercellular communication between microglia and the microenvironment. We previously showed that EVs produced under ATP stimulation (ATP-EVs) propagate a robust inflammatory reaction among astrocytes and microglia and in mice with subclinical neuroinflammation (Verderio et al., 2012). However, the proteome of EVs released upon ATP stimulation has not yet been elucidated. In this study we applied a label free proteomic approach to characterize the proteome of EVs released constitutively and during microglia activation with ATP. We show that ATP drives sorting in EVs of a set of proteins implicated in cell adhesion/extracellular matrix organization, autophagy-lysosomal pathway and cellular metabolism, that may influence the response of recipient astrocytes to EVs. These data provide new clues to molecular mechanisms involved in microglia response to ATP and in microglia signaling to the environment via EVs.

摘要

细胞外ATP是促进小胶质细胞激活并诱导细胞外囊泡（EVs）释放的分子之一，而细胞外囊泡是小胶质细胞与微环境之间细胞间通讯的有效介质。我们之前表明，在ATP刺激下产生的细胞外囊泡（ATP-EVs）在星形胶质细胞和小胶质细胞之间以及在亚临床神经炎症小鼠中引发强烈的炎症反应（Verderio等人，2012年）。然而，ATP刺激后释放的细胞外囊泡的蛋白质组尚未阐明。在本研究中，我们应用无标记蛋白质组学方法来表征在小胶质细胞组成性释放以及用ATP激活期间释放的细胞外囊泡的蛋白质组。我们表明，ATP驱动一组与细胞粘附/细胞外基质组织、自噬-溶酶体途径和细胞代谢相关的蛋白质进入细胞外囊泡，这可能会影响受体星形胶质细胞对细胞外囊泡的反应。这些数据为小胶质细胞对ATP的反应以及小胶质细胞通过细胞外囊泡向环境发出信号所涉及的分子机制提供了新线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/caf97eaf3bab/fphar-08-00910-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/1dd826959696/fphar-08-00910-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/7f2b520cbeb8/fphar-08-00910-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/d0131f04aa33/fphar-08-00910-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/5928ec09f29f/fphar-08-00910-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/f769829f39fb/fphar-08-00910-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/a7bc4d59d1eb/fphar-08-00910-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/caf97eaf3bab/fphar-08-00910-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/1dd826959696/fphar-08-00910-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/7f2b520cbeb8/fphar-08-00910-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/d0131f04aa33/fphar-08-00910-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e0/5733563/caf97eaf3bab/fphar-08-00910-g007.jpg

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2
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J Infect Public Health. 2017 Sep-Oct;10(5):649-656. doi: 10.1016/j.jiph.2017.05.008. Epub 2017 Jun 29.
3
Neurotoxic reactive astrocytes are induced by activated microglia.神经毒性反应性星形胶质细胞由活化的小胶质细胞诱导产生。
细胞外囊泡对突触线粒体的调控及其对神经元代谢和突触可塑性的影响。
J Cereb Blood Flow Metab. 2025 May 14:271678X251337630. doi: 10.1177/0271678X251337630.
4
Enhanced exosome secretion regulated by microglial P2X7R in the medullary dorsal horn contributes to pulpitis-induced pain.髓质背角中由小胶质细胞P2X7R调节的外泌体分泌增强促成了牙髓炎诱导的疼痛。
Cell Biosci. 2025 Feb 22;15(1):28. doi: 10.1186/s13578-025-01363-4.
5
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Int J Mol Sci. 2025 Feb 5;26(3):1345. doi: 10.3390/ijms26031345.
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Exp Hematol Oncol. 2025 Feb 1;14(1):12. doi: 10.1186/s40164-025-00603-0.
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