粗糙脉孢菌铜金属硫蛋白基因的分离与结构组织
Isolation and structural organization of the Neurospora crassa copper metallothionein gene.
作者信息
Münger K, Germann U A, Lerch K
出版信息
EMBO J. 1985 Oct;4(10):2665-8. doi: 10.1002/j.1460-2075.1985.tb03985.x.
Abstract
The Neurospora crassa copper metallothionein gene was cloned and its complete nucleotide sequence is reported. Enriched metallothionein mRNA was used as a template for cDNA synthesis, primed by a metallothionein-specific, synthetic undecanucleotide. The sequence of the cDNA obtained allowed the synthesis of a unique 21-mer which was used to screen a genomic DNA library of N. crassa. In agreement with the published amino acid sequence, the gene codes for a polypeptide 26 amino acid residues in length. The coding region is interrupted by a small intron (94 nucleotides). The gene structure is compared with those of mammalian metallothioneins. In both cases, the coding regions are split by introns, the intron-exon boundaries, however, are in different positions. The neurospora copper metallothionein gene is, to our knowledge, the smallest gene interrupted by an intron isolated so far.
摘要
克隆了粗糙脉孢菌的铜金属硫蛋白基因，并报道了其完整的核苷酸序列。以富集的金属硫蛋白mRNA为模板进行cDNA合成，由金属硫蛋白特异性的合成十一核苷酸引发。所获得的cDNA序列使得能够合成一种独特的21聚体，该21聚体用于筛选粗糙脉孢菌的基因组DNA文库。与已发表的氨基酸序列一致，该基因编码一个长度为26个氨基酸残基的多肽。编码区被一个小内含子（94个核苷酸）中断。将该基因结构与哺乳动物金属硫蛋白的基因结构进行了比较。在这两种情况下，编码区都被内含子分割，但内含子-外显子边界处于不同位置。据我们所知，粗糙脉孢菌铜金属硫蛋白基因是迄今为止分离出的被内含子中断的最小基因。
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