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多核苷酸的固相合成。III. 用片段偶联磷酸三酯法合成具有特定序列的多核苷酸。

Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.

作者信息

Miyoshi K, Huang T, Itakura K

出版信息

Nucleic Acids Res. 1980 Nov 25;8(22):5491-505. doi: 10.1093/nar/8.22.5491.

DOI:10.1093/nar/8.22.5491
PMID:7465419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324316/
Abstract

Preparation of the three hexadecanucleotides, dGpTpApTpCpApCpGpApGpGpCpCpCpTpT, dCpGpApCpGpApGpCpGpTpGpApCpApCpC and cTpGpCpCpGpGpCpCpApCpGpApTpGpCpG, is described by a rapid and simple solid-phase method on polyacrylamide supports. The synthesis were performed by the extension of the method described in the previous paper using di and trinucleotides of defined sequences as an incoming 3'-phosphodiester unit. Although the coupling yields to form phosphotriester bonds are slightly lower than those for the homothymidylic acid series, pure polydeoxyribonucleotides of defined sequences can be synthesized without any major difficulty.

摘要

三种十六聚核苷酸dGpTpApTpCpApCpGpApGpGpCpCpCpTpT、dCpGpApCpGpApGpCpGpTpGpApCpApCpC和cTpGpCpCpGpGpCpCpApCpGpApTpGpCpG的制备方法，采用了在聚丙烯酰胺载体上快速简便的固相法进行描述。合成是通过扩展前一篇论文中描述的方法来完成的，使用特定序列的二核苷酸和三核苷酸作为引入的3'-磷酸二酯单元。尽管形成磷酸三酯键的偶联产率略低于同胸腺嘧啶酸系列，但可以毫无重大困难地合成特定序列的纯聚脱氧核糖核苷酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/f3a565924e9e/nar00439-0362-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/f7705f263576/nar00439-0361-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/fcf37134f096/nar00439-0361-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/f3a565924e9e/nar00439-0362-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/f7705f263576/nar00439-0361-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/fcf37134f096/nar00439-0361-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf60/324316/f3a565924e9e/nar00439-0362-a.jpg

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Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.多核苷酸的固相合成。III. 用片段偶联磷酸三酯法合成具有特定序列的多核苷酸。
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本文引用的文献

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DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
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Nucleic Acids Res. 1980 Nov 25;8(22):5473-89. doi: 10.1093/nar/8.22.5473.
3
Simplications in the synthesis of short oligonucleotide blocks.
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Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.人β-珠蛋白基因的寡核苷酸定向诱变：在克隆DNA中产生特定位点突变的通用方法。
Nucleic Acids Res. 1981 Aug 11;9(15):3647-56. doi: 10.1093/nar/9.15.3647.
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Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.
6
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