Zhu Zhen, Tsuchimoto Maho, Nagata Shinji
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
Insects. 2022 Mar 25;13(4):324. doi: 10.3390/insects13040324.
Neuropeptides play vital roles in energy homeostasis in insects and other animals. Although the importance of the regulatory network of neuropeptides in feeding and metabolism has been illuminated, a complete understanding of the mechanisms has not been addressed as many factors are involved in the regulation. CCHamide-2 is a newly identified brain-gut neuropeptide that regulates feeding behavior in several insect species including . However, little is known about the mechanisms controlling the feeding-related behavior and metabolic functions modulated by CCHamide-2 in other insects. In this study, we addressed the functions of CCHamide-2 in the two-spotted cricket, , which was used as the experimental material to research the mechanisms of feeding and metabolism in this omnivorous insect species. Knockdown crickets by RNA interference against increased the amount of food intake, while injection of chemically synthetic GbCCHamide-2 peptide reduced the amount of food intake. Further, knockdown and peptide injection experiments revealed that GbCCHamide-2 signaling increased the concentrations of circulating lipids and carbohydrates, and the carbohydrate-rich diet increased the transcript levels of Moreover, GbCCHa-2 injection decreased the transcript level of . By contrast, GbCCHamide-2 signaling did not affect nymphal growth or the transcript level of , as well as feeding preferences. Taken together, CCHamide-2 signaling in regulates food intake associated with alterations in lipid and carbohydrate levels in hemolymph.
神经肽在昆虫和其他动物的能量平衡中起着至关重要的作用。尽管神经肽调节网络在摄食和新陈代谢中的重要性已得到阐明，但由于该调节涉及许多因素，尚未完全了解其机制。CCHamide-2是一种新发现的脑-肠神经肽，可调节包括[昆虫名称未给出]在内的几种昆虫的摄食行为。然而，对于CCHamide-2在其他昆虫中调控与摄食相关行为和代谢功能的机制知之甚少。在本研究中，我们研究了CCHamide-2在双斑蟋蟀[昆虫名称未给出]中的功能，该昆虫被用作实验材料来研究这种杂食性昆虫的摄食和代谢机制。通过RNA干扰敲低[基因名称未给出]的蟋蟀增加了食物摄入量，而注射化学合成的GbCCHamide-2肽则减少了食物摄入量。此外，敲低和肽注射实验表明，GbCCHamide-2信号增加了循环脂质和碳水化合物的浓度，富含碳水化合物的饮食增加了[基因名称未给出]的转录水平。此外，注射GbCCHa-2降低了[基因名称未给出]的转录水平。相比之下，GbCCHamide-2信号不影响若虫生长或[基因名称未给出]的转录水平以及摄食偏好。综上所述，双斑蟋蟀[昆虫名称未给出]中的CCHamide-2信号调节与血淋巴中脂质和碳水化合物水平变化相关的食物摄入量。
