两种药用和芳香植物（阿米茴芹和石榴）对一水合草酸钙结晶抑制作用的研究。

The study of the inhibitory effect of calcium oxalate monohydrate's crystallization by two medicinal and aromatic plants: Ammi visnaga and Punica granatum.

作者信息

Kachkoul R, Sqalli Houssaini T, Miyah Y, Mohim M, El Habbani R, Lahrichi A

机构信息

Laboratory of biochemistry, faculty of medicine and pharmacy, university Sidi Mohammed Ben Abdellah, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco.

Department of nephrology, university hospital Hassan II, BP 1835, Atlas, Road of Sidi Harazem, Fez, Morocco; Laboratory of molecular bases in human pathology and therapeutic tools, faculty of medicine and pharmacy, university Sidi Mohammed Ben Abdellah, BP 1893, Km 22, Road of Sidi Harazem, Fez, Morocco.

出版信息

Prog Urol. 2018 Mar;28(3):156-165. doi: 10.1016/j.purol.2017.12.003. Epub 2018 Jan 9.

DOI:10.1016/j.purol.2017.12.003

PMID:29325926

Abstract

INTRODUCTION

Urinary lithiasis is a recurrent disease defined by the presence of calculi in the urinary tract. Most urinary calculi have as a major component calcium oxalate which occurs mainly in two crystalline forms: Calcium oxalate monohydrate (whewellite) and calcium oxalate dihydrate (weddellite). The target behind, this work is to study the inhibiting effect of the calcium oxalate's crystallization by the extract of the Ammi visnaga and the Punica granatum.

METHODS

The inhibition of crystallization has been studied in vitro with both the absence and the presence of the different concentrations of the extracts of the two plants. This study consists in measurement, with the UV-Visible spectrophotometer, the temporal evolution of the optical density at λ equal to 620nm corresponding to the formation of the crystals due to the mixing of metastable solutions of calcium and oxalate. The characterization of the crystals is carried out in parallel by both the Fourier transform infrared spectra (FT-IR) and the observation of the crystals with the help of an optical microscope. In this respect, the inhibition percentages were calculated from the turbidity slopes in the presence and absence of the extract.

RESULTS

The results obtained were more effective, especially for Punica granatum with percentages of 97.8±0.12 and 83.46±1.34% against nucleation and aggregation, respectively, the order of Ammi visnaga was as follow: 73.25±0.81 and 59.44±3.3%. Thus, all correlation coefficients are greater than 0.95 and all coefficients of variation are less than 10%.

CONCLUSIONS

The prevention and treatment of urinary lithiasis and especially in the case of recurrence by plants remains an alternative choice for medical methods. This study justified the efficacy of the plants Ammi visnaga and in particular Punica granatum against the crystallization of calcium oxalate.

LEVEL OF EVIDENCE

摘要

引言

尿路结石是一种由尿路中存在结石所定义的复发性疾病。大多数尿路结石的主要成分是草酸钙，其主要以两种晶体形式存在：一水合草酸钙（文石）和二水合草酸钙（水草酸钙石）。这项工作的目标是研究阿米芹和石榴提取物对草酸钙结晶的抑制作用。

方法

在有和没有两种植物不同浓度提取物的情况下，体外研究了结晶抑制情况。该研究包括用紫外可见分光光度计测量在λ等于620nm处的光密度随时间的变化，这对应于由于钙和草酸盐的亚稳溶液混合而形成晶体的过程。同时通过傅里叶变换红外光谱（FT-IR）以及借助光学显微镜观察晶体来对晶体进行表征。在这方面，根据有提取物和无提取物时的浊度斜率计算抑制百分比。