Institute of Horticultural Production Systems, Section Phytomedicine, Leibniz University Hannover, Herrenhäuser Str. 2, 30419, Hannover, Germany.
Arch Virol. 2018 Apr;163(4):1107-1111. doi: 10.1007/s00705-018-3705-1. Epub 2018 Jan 11.
The complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZ PV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californian isolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typical organization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from different origins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531.1) and Aschersleben, Germany (AJ271087.1) show a NAG instead of DAG in the region of the coat protein responsible for aphid transmission. In this study the first infectious full-length clone of celery mosaic virus was obtained and the infectivity confirmed by Rhizobium radiobacter infiltration of Apium species.
从德国屈德林堡（DSMZ PV-1003）分离得到的芹菜花叶病毒（CeMV，一种马铃薯 Y 病毒属病毒）的完整基因组序列已被测定（MF962880）。这是该病毒的第二个全序列基因组，与加利福尼亚分离株（HQ676607.1）一起。正链单链 RNA 长 10000 个核苷酸，表现出马铃薯 Y 病毒属病毒的典型结构，但 PIPO 比加利福尼亚分离株 CeMV 短。与来自不同起源的 CeMV 分离株相比，CeMV-屈德林堡和来自荷兰（AF203531.1）和德国阿舍斯莱本（AJ271087.1）的分离株在负责蚜虫传播的外壳蛋白区域显示 NAG 而不是 DAG。在本研究中，首次获得了具有感染性的芹菜花叶病毒全长克隆，并通过 Rhizobium radiobacter 对芹菜属物种的渗透证实了其感染性。
