用于鉴定肺炎链球菌分离株的“Xisco”基因检测
Detection of "Xisco" gene for identification of Streptococcus pneumoniae isolates.
作者信息
Salvà-Serra Francisco, Connolly Gwendolyn, Moore Edward R B, Gonzales-Siles Lucia
机构信息
Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Culture Collection University of Gothenburg (CCUG), Sahlgrenska Academy, University of Gothenburg, Sweden; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden; Microbiology, Department of Biology, University of the Balearic Islands, Palma de Mallorca, Spain.
Culture Collection University of Gothenburg (CCUG), Sahlgrenska Academy, University of Gothenburg, Sweden; School of Biological Sciences, Dublin Institute of Technology, Dublin, Ireland.
出版信息
Diagn Microbiol Infect Dis. 2018 Apr;90(4):248-250. doi: 10.1016/j.diagmicrobio.2017.12.003. Epub 2017 Dec 15.
We describe a PCR-assay differentiating Streptococcus pneumoniae from closely-related species of the Mitis group of the genus Streptococcus and identification of pneumococcus clinical isolates, based on the "Xisco" gene discriminatory marker. The complete "Xisco" gene sequence was observed in all S. pneumoniae genomes analyzed and absent in all non-pneumococcus genomes.
我们描述了一种基于“Xisco”基因鉴别标记的聚合酶链反应（PCR）检测方法，该方法可区分肺炎链球菌与链球菌属米氏菌群的密切相关物种，并对肺炎球菌临床分离株进行鉴定。在所分析的所有肺炎链球菌基因组中均观察到完整的“Xisco”基因序列，而在所有非肺炎球菌基因组中均未发现该序列。
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