Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India.
Division of Veterinary Pharmacology and Toxicology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, India.
Environ Sci Pollut Res Int. 2018 Mar;25(9):8853-8860. doi: 10.1007/s11356-018-1216-y. Epub 2018 Jan 12.
Adenosine triphosphate-binding cassette (ABC) sub-family G member-2 (ABCG-2) is a transporter protein, implicated for multi-drug efflux from tissues. This study evaluated the effect of fluoroquinolones; levofloxacin, pazufloxacin and enrofloxacin, and non-steroidal anti-inflammatory drug, meloxicam; on the immunolocalization of ABCG-2 transporter protein of rabbit retinas. Thirty-two male rabbits were randomly divided in to eight groups. Control group was gavaged, 2% benzyl alcohol in 5% dextrose since these chemicals are excipients of the drug preparations used in the treatment groups of this study. Four groups were exclusively gavaged, levofloxacin hemihydrate (10 mg/kg body weight b.i.d 12 h), pazufloxacin mesylate (10 mg/kg body weight b.i.d 12 h), enrofloxacin (20 mg/kg body weight o.d.), and meloxicam (0.2 mg/kg body weight o.d.), respectively. Three other groups were co-gavaged meloxicam with above fluoroquinolones, respectively. These drugs were administered for 21 days. ABCG-2 immunolocalization was mild in the retinas of control and levofloxacin-alone-treated groups. The immunolocalization intensity was significantly higher in meloxicam-alone-treated group when compared to control and levofloxacin-alone-treated groups. Immunolocalization of this transporter increased in the levofloxacin-meloxicam co-treated group when compared to the levofloxacin-alone-treated group. Highest immunolocalization was observed in the enrofloxacin-meloxicam co-treated group although the immunolocalization of all treatment groups, except the levofloxacin-alone-treated group, was significantly higher than the control and levofloxacin-alone-treated groups.
三磷酸腺苷结合盒（ABC）亚家族 G 成员 2（ABCG-2）是一种转运蛋白，与组织中的多药外排有关。本研究评估了氟喹诺酮类药物；左氧氟沙星、帕珠沙星和恩诺沙星，以及非甾体抗炎药；美洛昔康对兔视网膜 ABCG-2 转运蛋白免疫定位的影响。32 只雄性兔子被随机分为 8 组。对照组灌胃，2%苯甲醇在 5%葡萄糖中，因为这些化学物质是本研究治疗组药物制剂的赋形剂。四组专门灌胃，左氧氟沙星一水合物（10mg/kg 体重，bid 12h），甲磺酸帕珠沙星（10mg/kg 体重，bid 12h），恩诺沙星（20mg/kg 体重，od），和美洛昔康（0.2mg/kg 体重，od），分别。另外三组分别与上述氟喹诺酮类药物共同灌胃美洛昔康。这些药物给药 21 天。与单独使用左氧氟沙星治疗组相比，对照组和单独使用左氧氟沙星治疗组的视网膜 ABCG-2 免疫定位较轻。与对照组和单独使用左氧氟沙星治疗组相比，单独使用美洛昔康治疗组的免疫定位强度显著更高。与单独使用左氧氟沙星治疗组相比，左氧氟沙星-美洛昔康联合治疗组的这种转运蛋白的免疫定位增加。在恩诺沙星-美洛昔康联合治疗组中观察到最高的免疫定位，尽管所有治疗组（除单独使用左氧氟沙星治疗组外）的免疫定位均明显高于对照组和单独使用左氧氟沙星治疗组。
