de Oliveira Guilherme J P L, Aroni Maurício A T, Medeiros Marcell C, Marcantonio Elcio, Marcantonio Rosemary A C
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, State University of Sao Paulo (Universidade Estadual Paulista-UNESP), Humaitá st.1680, Araraquara, São Paulo, 14801-930, Brazil.
Lasers Surg Med. 2018 Jan 13. doi: 10.1002/lsm.22787.
The aim of this study was to evaluate the effect of low-level laser therapy (LLLT) on the healing of biomaterial graft areas (i.e., coagulum, deproteinized bovine bone, and biphasic ceramics comprising hydroxyapatite and β-tricalcium phosphate).
Ninety rats were divided into two groups according to laser irradiation use (λ 808 nm, 100 mW, φ ∼600 μm, seven sessions with 28 J of irradiation dose in total): a laser group and a control group. Each of these groups was divided into three subgroups of 15 animals each according to the type of biomaterial used: Coagulum (COA), deproteinized bovine bone (DBB), and hydroxyapatite/β-tricalcium phosphate (HA/βTCP). Biomaterials were inserted into Teflon domes, and these domes were grafted to the lateral aspect of the mandibular branch of the rats. The animals were sacrificed after 30, 60, and 90 days. Scarring patterns were evaluated by microtomography and histometry. The expression levels of BMP2, osteocalcin (OCN), and alkaline phosphatase (ALP) were evaluated by immunohistochemistry. The mRNA expression levels of ALP, BMP2, Jagged1, Osterix, Runx2, and TGFβ1 were determined by RT-qPCR.
The animals treated with LLLT exhibited increased mineralized tissues and bone, particularly after 90 days. These increases were associated with increased BMP2, OCN, and ALP protein expression and ALP, BMP2, and Jagged1 mRNA expression.
LLLT improved the osteoconductive potential of DBB and HA/βTCP grafts and bone formation in ungrafted areas. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.
本研究旨在评估低强度激光疗法（LLLT）对生物材料移植区域（即凝块、脱蛋白牛骨以及由羟基磷灰石和β-磷酸三钙组成的双相陶瓷）愈合的影响。
根据是否使用激光照射（波长808nm，功率100mW，光斑直径约600μm，共七次照射，总照射剂量28J）将90只大鼠分为两组：激光组和对照组。每组再根据所用生物材料类型分为三个亚组，每组15只动物：凝块（COA）、脱蛋白牛骨（DBB）和羟基磷灰石/β-磷酸三钙（HA/βTCP）。将生物材料植入特氟龙穹顶中，并将这些穹顶移植到大鼠下颌支外侧。分别在30天、60天和90天后处死动物。通过显微断层扫描和组织计量学评估瘢痕形成模式。通过免疫组织化学评估骨形态发生蛋白2（BMP2）、骨钙素（OCN）和碱性磷酸酶（ALP）的表达水平。通过逆转录定量聚合酶链反应（RT-qPCR）测定ALP、BMP2、锯齿状蛋白1（Jagged1）、osterix、Runx2和转化生长因子β1（TGFβ1）的mRNA表达水平。
接受LLLT治疗的动物矿化组织和骨量增加，尤其是在90天后。这些增加与BMP2、OCN和ALP蛋白表达以及ALP、BMP2和Jagged1 mRNA表达增加有关。
LLLT提高了DBB和HA/βTCP移植物的骨传导潜力以及未移植区域的骨形成。《激光外科与医学》。©2018威利期刊公司。
