特应性和非特应性受试者的同种异体T细胞在体外对IgE合成的抑制作用。
Suppression of IgE synthesis in vitro by allogeneic T cells from atopic and non-atopic subjects.
作者信息
Zaunders J J, Cooper D A, Young Y, Duckett M, Penny R, Ziegler J B
出版信息
Clin Exp Immunol. 1985 Oct;62(1):193-9.
The role of T cells in the regulation of IgE synthesis by human PBMC was studied. PBMC or separated and recombined populations of T and B cells from both normal and atopic donors were cultured for 10 days with and without cycloheximide. IgE and IgG synthesis were determined by specific RIA. IgE synthesis was detected in 0/30 non-atopic, 6/34 mildly atopic and 25/31 severely atopic subjects. Autologous T cells from 10/26 atopic donors, whose B cells synthesised IgE, significantly suppressed this IgE synthesis. The addition of allogeneic T cells from atopic or non-atopic subjects to atopic B cells resulted in greater suppression of IgE synthesis than the addition of autologous T cells. These data support the notion that atopic subjects have naturally occurring IgE isotype-specific suppressor T cells as well as suppressor T cells which can be activated during incubation with alloantigen.
研究了T细胞在人外周血单核细胞（PBMC）调节IgE合成中的作用。将来自正常和特应性供体的PBMC或分离并重组的T细胞和B细胞群体在有或无环己酰亚胺的情况下培养10天。通过特异性放射免疫分析（RIA）测定IgE和IgG的合成。在30名非特应性受试者中未检测到IgE合成，在34名轻度特应性受试者中有6名检测到，在31名重度特应性受试者中有25名检测到。来自26名特应性供体中的10名的自体T细胞，其B细胞合成IgE，显著抑制了这种IgE合成。将来自特应性或非特应性受试者的同种异体T细胞添加到特应性B细胞中，比添加自体T细胞对IgE合成的抑制作用更大。这些数据支持这样的观点，即特应性受试者具有天然存在的IgE同种型特异性抑制性T细胞以及在与同种异体抗原孵育期间可被激活的抑制性T细胞。
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