Turner K J, Holt B J, Holt P G
Clin Exp Immunol. 1981 Mar;43(3):458-62.
A microculture system was developed for in vitro IgE synthesis employing human peripheral blood leucocytes (PBL), comprising 1.0 x 10(6) cells in 1.0 ml serum-supplemented medium, in round-bottomed tubes. Examination of the kinetics of IgE synthesis indicated that production was relatively linear during culture for up to 12 days, and was not susceptible to stimulation with polyclonal B cell mitogen. Employing a standardized 7-day culture period, PBL IgE synthesis was compared within a group of 50 volunteers, comprising normal non-atopic individuals exhibiting serum IgE titres less than 50 units/ml, and moderate atopics with serum titres up to approximately 400 units/ml. PBL IgE synthesis was found to reflect closely the serum IgE status of the cell donor, a feature thus far unique to this culture system, and indicative of the preservation of normal in vivo regulatory mechanisms in the in vitro cultures.
开发了一种用于体外 IgE 合成的微培养系统，该系统采用人外周血白细胞（PBL），在圆底管中，1.0 ml 补充血清的培养基中含有 1.0×10⁶ 个细胞。对 IgE 合成动力学的研究表明，在长达 12 天的培养过程中，产量相对呈线性，并且不易受到多克隆 B 细胞有丝分裂原的刺激。采用标准化的 7 天培养期，在一组 50 名志愿者中比较了 PBL IgE 的合成情况，这组志愿者包括血清 IgE 滴度低于 50 单位/ml 的正常非特应性个体和血清滴度高达约 400 单位/ml 的中度特应性个体。发现 PBL IgE 的合成与细胞供体的血清 IgE 状态密切相关，这是该培养系统迄今为止独有的特征，表明体外培养中体内正常调节机制得以保留。
