ICAR-Indian Institute of Pulses Research (IIPR), Kanpur, 208024, India.
Plant Cell Rep. 2018 Feb;37(2):177-191. doi: 10.1007/s00299-018-2248-y. Epub 2018 Jan 13.
Latest outcomes assign functional role to non-coding (nc) RNA molecules in regulatory networks that confer male sterility to plants. Male sterility in plants offers great opportunity for improving crop performance through application of hybrid technology. In this respect, cytoplasmic male sterility (CMS) and sterility induced by photoperiod (PGMS)/temperature (TGMS) have greatly facilitated development of high-yielding hybrids in crops. Participation of non-coding (nc) RNA molecules in plant reproductive development is increasingly becoming evident. Recent breakthroughs in rice definitively associate ncRNAs with PGMS and TGMS. In case of CMS, the exact mechanism through which the mitochondrial ORFs exert influence on the development of male gametophyte remains obscure in several crops. High-throughput sequencing has enabled genome-wide discovery and validation of these regulatory molecules and their target genes, describing their potential roles performed in relation to CMS. Discovery of ncRNA localized in plant mtDNA with its possible implication in CMS induction is intriguing in this respect. Still, conclusive evidences linking ncRNA with CMS phenotypes are currently unavailable, demanding complementing genetic approaches like transgenics to substantiate the preliminary findings. Here, we review the recent literature on the contribution of ncRNAs in conferring male sterility to plants, with an emphasis on microRNAs. Also, we present a perspective on improved understanding about ncRNA-mediated regulatory pathways that control male sterility in plants. A refined understanding of plant male sterility would strengthen crop hybrid industry to deliver hybrids with improved performance.
最新的研究结果赋予了非编码（nc）RNA 分子在调控网络中发挥功能的作用，这些调控网络赋予植物雄性不育。植物雄性不育为通过杂种技术应用提高作物性能提供了巨大的机会。在这方面，细胞质雄性不育（CMS）和光周期（PGMS）/温度（TGMS）诱导的不育极大地促进了作物高产杂种的发展。非编码（nc）RNA 分子参与植物生殖发育的作用越来越明显。最近在水稻中的突破明确将 ncRNA 与 PGMS 和 TGMS 相关联。在 CMS 的情况下，在线粒体 ORFs 对雄性配子体发育产生影响的确切机制在几种作物中仍然不清楚。高通量测序使这些调控分子及其靶基因的全基因组发现和验证成为可能，描述了它们在 CMS 中发挥的潜在作用。在这方面，发现定位于植物 mtDNA 中的 ncRNA 及其可能在 CMS 诱导中的作用令人着迷。尽管如此，目前还没有将 ncRNA 与 CMS 表型联系起来的确凿证据，需要补充遗传方法（如转基因）来证实初步发现。在这里，我们回顾了关于 ncRNA 赋予植物雄性不育的最新文献，重点介绍了 microRNA。此外，我们还介绍了对 ncRNA 介导的调控途径在植物雄性不育中控制作用的理解的展望。对植物雄性不育的更深入理解将加强作物杂种产业，以提供具有改进性能的杂种。
