Udagawa Sayuri, Katagiri Sayaka, Maekawa Shogo, Takeuchi Yasuo, Komazaki Rina, Ohtsu Anri, Sasaki Naoki, Shiba Takahiko, Watanabe Kazuki, Ishihara Kazuyuki, Sato Noriko, Miyasaka Naoyuki, Izumi Yuichi
a Department of Periodontology, Graduate School of Medical and Dental Sciences , Tokyo Medical and Dental University , Tokyo , Japan.
b Oral Health Science Center , Tokyo Dental College , Tokyo , Japan.
Acta Odontol Scand. 2018 Aug;76(6):433-441. doi: 10.1080/00016357.2018.1426876. Epub 2018 Jan 15.
Growing evidence indicates an association between periodontitis and delivery outcome; however, the mechanism is unclear. This study aimed to investigate the influence of Porphyromonas gingivalis (Pg) infection on delivery outcome in mice.
Bacteremia was induced in pregnant Slc:ICR mice (8 weeks old) by intravenous injection of Pg. Mice were randomly divided into a control group (CO), and those receiving Pg injection at gestational day 1 (GD1), gestational day 15 (GD15) or every day (ED). Delivery outcome, Pg infection, and gene expression in the placenta and umbilical cord were evaluated.
Birth weight was lower in the ED and GD15 groups than in the CO group. A remarkable increase in anti-Pg IgG antibody was observed in the ED and GD1 groups, although Pg was not detected in the placenta or umbilical cord. mRNA expression of Tnfα and Il6 in the placenta, and Hif1α in the umbilical cord, was significantly increased in the ED group. Microarray analysis of the umbilical cord revealed increased expression of several genes including Orm1, Mgl2, Rps6ka3 and Trim15 in the ED group.
Pg infection during the third trimester caused low birth weight and inflammation in the placenta and umbilical cord.
越来越多的证据表明牙周炎与分娩结局之间存在关联；然而，其机制尚不清楚。本研究旨在探讨牙龈卟啉单胞菌（Pg）感染对小鼠分娩结局的影响。
通过静脉注射Pg在怀孕的Slc:ICR小鼠（8周龄）中诱导菌血症。小鼠被随机分为对照组（CO），以及在妊娠第1天（GD1）、妊娠第15天（GD15）或每天（ED）接受Pg注射的组。评估分娩结局、Pg感染以及胎盘和脐带中的基因表达。
ED组和GD15组的出生体重低于CO组。尽管在胎盘或脐带中未检测到Pg，但在ED组和GD1组中观察到抗Pg IgG抗体显著增加。ED组中胎盘的Tnfα和Il6以及脐带的Hif1α的mRNA表达显著增加。脐带的微阵列分析显示ED组中包括Orm1、Mgl2、Rps6ka3和Trim15在内的几种基因的表达增加。
妊娠晚期的Pg感染导致低出生体重以及胎盘和脐带炎症。
