School of Medical Science and Technology, IIT Kharagpur, West Bengal, India.
Department of Electronics and Communications Engineering, DSPM IIIT, Naya Raipur, India.
Sci Rep. 2018 Jan 15;8(1):783. doi: 10.1038/s41598-017-18965-9.
Determination of cancer aggressiveness is mainly assessed in tissues by looking at the grade of cancer. There is a lack of specific method to determine aggressiveness of cancer cells in vitro. In our present work, we have proposed a bio-impedance based non-invasive method to differentiate aggressive property of two breast cancer cell lines. Real-time impedance analysis of MCF-7 (less aggressive) and MDA-MB-231 cells (more aggressive) demonstrated unique growth pattern. Detailed slope-analysis of impedance curves at different growth phases showed that MDA-MB-231 had higher proliferation rate and intrinsic resistance to cell death, when allowed to grow in nutrient and space limiting conditions. This intrinsic nature of death resistance of MDA-MB-231 was due to modulation and elongation of filopodia, which was also observed during scanning electron microscopy. Results were also similar when validated by cell cycle analysis. Additionally, wavelet based analysis was used to demonstrate that MCF-7 had lesser micromotion based cellular activity, when compared with MDA-MB-231. Combined together, we hypothesize that analysis of growth rate, death resistance and cellular energy, through bioimpedance based analysis can be used to determine and compare aggressiveness of multiple cancer cell lines. This further opens avenues for extrapolation of present work to human tumor tissue samples.
癌症侵袭性的测定主要通过观察癌症的分级在组织中进行评估。目前缺乏一种特定的方法来确定体外癌细胞的侵袭性。在本研究中，我们提出了一种基于生物阻抗的非侵入性方法，以区分两种乳腺癌细胞系的侵袭特性。MCF-7（侵袭性较低）和 MDA-MB-231 细胞（侵袭性较高）的实时阻抗分析显示出独特的生长模式。在不同生长阶段的阻抗曲线的详细斜率分析表明，在营养和空间有限的条件下允许生长时，MDA-MB-231 具有更高的增殖率和内在的抗细胞死亡能力。MDA-MB-231 的这种内在抗死亡特性是由于丝状伪足的调制和伸长，这在扫描电子显微镜下也观察到了。通过细胞周期分析验证，结果也相似。此外，基于小波的分析表明，与 MDA-MB-231 相比，MCF-7 的基于微运动的细胞活性更小。综上所述，我们假设通过基于生物阻抗的分析来测定和比较多种癌细胞系的侵袭性，可以用来测定和比较多种癌细胞系的侵袭性。这进一步为将目前的工作推广到人类肿瘤组织样本开辟了道路。
