Glycometabolome Team, Systems Glycobiology Research Group, Global Research Cluster, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura, Saitama, 338-8570, Japan.
Glycoconj J. 2018 Apr;35(2):165-176. doi: 10.1007/s10719-017-9807-2. Epub 2018 Jan 15.
C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excreted via the urine. This paper reports enrichment of a bacterial consortium from soil that degrades CMW. The bacteria grew in minimal medium supplemented with CMW as the carbon source. Interestingly, even after successive clonal picks of individual colonies, several species were still present in each colony as revealed by 16S rRNA gene sequence analysis, indicating that a single species may not be responsible for this activity. A next generation sequencing (NGS) analysis was therefore carried out in order to determine which bacteria were responsible for the catabolism of CMW. It was found that a species of Sphingomonadaceae family, but not others, increased with simultaneous decrease of CMW in the media, suggesting that this species is most likely the one that is actively involved in the degradation of CMW.
C-甘露糖化，一种在各种真核生物中发现的蛋白质修饰，涉及到将一个甘露糖分子连接到蛋白质中选定的色氨酸残基上。由于在人尿中检测到 C-甘露糖基色氨酸（CMW），因此普遍认为 CMW 在体内不会被代谢，而是通过尿液排出体外。本文报道了从土壤中富集能够降解 CMW 的细菌联合体。这些细菌在含有 CMW 作为碳源的最小培养基中生长。有趣的是，即使对单个菌落进行连续的克隆挑选，每个菌落中仍存在多种物种，这一点通过 16S rRNA 基因序列分析得到了揭示，表明可能不是单个物种负责这种活性。因此，进行了下一代测序（NGS）分析，以确定哪些细菌负责 CMW 的代谢。结果发现，一种鞘氨醇单胞菌科的细菌，但不是其他细菌，随着培养基中 CMW 的同时减少而增加，这表明该物种很可能是积极参与 CMW 降解的物种。
