Haque A, Cuna W, Bonnel B, Capron A, Joseph M
Parasite Immunol. 1985 Sep;7(5):517-26. doi: 10.1111/j.1365-3024.1985.tb00096.x.
The platelets from normal rats interact with microfilariae of Dipetalonema viteae in vitro in the presence of antibodies leading to the killing of the parasite. The antibody involved in this reaction is identified as IgE because the absorption of immune rat serum on anti-rat IgE column or the pretreatment of platelets with anti-Fc epsilon receptor resulted in a significant reduction in the percentage of killing of microfilariae. This antibody, which mediates platelet activity towards microfilariae, appears early in the secondary infection and persists for a short period of time. This short-lasting IgE antibody is not apparently present in the form of large complexes since the supernatant but not the pellet after ultracentrifugation was able to mediate killing of microfilariae by platelets. IgE-dependent platelet-mediated parasite killing is neither stage- nor species-specific because the microfilariae (LI) of Brugia malayi or of Loa loa and infective larvae (L3) of D. viteae or of B. malayi were killed when they were incubated with the serum obtained from rats at day 8 after secondary infection with adult D. viteae worms. The results of the present study suggest that platelets can actively participate in the immunological killing of filarial larvae.
在抗体存在的情况下，正常大鼠的血小板在体外与魏氏双瓣线虫的微丝蚴相互作用，导致寄生虫被杀死。参与该反应的抗体被鉴定为IgE，因为免疫大鼠血清在抗大鼠IgE柱上的吸附或用抗Fcε受体对血小板进行预处理会导致微丝蚴杀伤百分比显著降低。这种介导血小板对微丝蚴活性的抗体在二次感染早期出现，并持续较短时间。这种短暂存在的IgE抗体显然不是以大复合物的形式存在，因为超速离心后的上清液而非沉淀能够介导血小板对微丝蚴的杀伤。IgE依赖的血小板介导的寄生虫杀伤既不是阶段特异性也不是物种特异性的，因为当马来布鲁线虫或罗阿丝虫的微丝蚴（L1）以及魏氏双瓣线虫或马来布鲁线虫的感染性幼虫（L3）与二次感染成年魏氏双瓣线虫后第8天的大鼠血清一起孵育时，它们会被杀死。本研究结果表明，血小板可积极参与丝虫幼虫的免疫杀伤。
