Aime N, Haque A, Bonnel B, Torpier G, Capron A
Immunology. 1984 Mar;51(3):585-94.
Neutrophils from the peripheral washings of normal rats in the presence of sera obtained from rats immune to circulating microfilariae adhered to and killed the microfilariae of Dipetalonema viteae in vitro within 16-24 hr. No significant adherence or cytotoxicity was mediated by sera collected from animals with a high microfilaraemia or from normal rats. Ultrastructural studies show that neutrophils, which are bigger than microfilariae, can easily internalize the small larvae resulting in the disintegration of the parasite. Immunoadsorption and inhibition experiments showed that the adherence-promoting activity resides both in IgG and IgE classes of antibody. However, the mere participation of these two antibodies is not sufficient to effect neutrophil adherence towards microfilariae, the presence of complement is also required. Samples of fresh immune rat serum (fIRS) depleted in alternative pathway components of complement by treatment with zymosan A failed to mediate cell adherence to the parasite. fIRS inactivated for the classical pathway of complement by the chelating agent EGTA partially retains its activity in mediating cytotoxicity to microfilariae. The striking antigenic specificity of D. viteae antibodies was shown by their ability to mediate cytotoxicity only to D. viteae but not towards Brugia malayi microfilariae.
在存在从对循环微丝蚴免疫的大鼠获得的血清的情况下，正常大鼠外周洗液中的中性粒细胞在体外16 - 24小时内黏附并杀死了魏氏棘唇线虫的微丝蚴。从高微丝蚴血症动物或正常大鼠收集的血清未介导显著的黏附或细胞毒性。超微结构研究表明，比微丝蚴大的中性粒细胞能够轻易地内化小幼虫，导致寄生虫解体。免疫吸附和抑制实验表明，促进黏附的活性存在于IgG和IgE类抗体中。然而，仅这两种抗体的参与不足以使中性粒细胞黏附于微丝蚴，还需要补体的存在。用酵母聚糖A处理使补体替代途径成分耗竭的新鲜免疫大鼠血清（fIRS）样本未能介导细胞对寄生虫的黏附。经螯合剂EGTA使补体经典途径失活的fIRS在介导对微丝蚴的细胞毒性方面部分保留了其活性。魏氏棘唇线虫抗体显著的抗原特异性表现为它们仅对魏氏棘唇线虫微丝蚴具有介导细胞毒性的能力，而对马来布鲁线虫微丝蚴则无此能力。
