Chair of Medical Biotechnology, Poznan University of Medical Sciences, 61-688 Poznan, Poland.
NanoBioMedical Centre, Adam Mickiewicz University, 61-614 Poznan, Poland.
Nanomedicine (Lond). 2018 Feb;13(4):439-454. doi: 10.2217/nnm-2017-0276. Epub 2018 Jan 17.
Analysis of the properties and chemotherapeutics delivery potential of spheres made of bioengineered spider silks MS1 and MS2.
MATERIALS & METHODS: MS1 and MS2 derived from Nephila clavipes dragline silks - MaSp1 and MaSp2, respectively - formed spheres that were compared in terms of physicochemical properties, cytotoxicity and loading/release of chemotherapeutics.
MS2 spheres were more dispersed, smaller, of solid core, of higher beta-sheet structure content, and of opposite (negative) charge than MS1 spheres. Preloaded MS2 showed greater applicability for mitoxantrone, while postloaded for etoposide delivery compared with MS1 spheres. However, MS1 spheres were a better choice for doxorubicin delivery than MS2.
Bioengineered silks can be tailored to develop a system with optimal drug loading and release properties.
分析由生物工程蛛丝 MS1 和 MS2 制成的球体的特性和化疗药物传递潜力。
MS1 和 MS2 分别来自金蛛牵引丝 MaSp1 和 MaSp2，形成了球体，从理化性质、细胞毒性以及化疗药物的负载/释放方面对它们进行了比较。
MS2 球体更分散、更小、为实心核、β-折叠结构含量更高、带相反（负）电荷，而 MS1 球体则相反。与 MS1 球体相比，载有米托蒽醌的 MS2 球体具有更高的适用性，而载有依托泊苷的 MS1 球体则具有更高的适用性。然而，MS1 球体在阿霉素传递方面优于 MS2 球体。
生物工程丝可以进行定制，以开发出具有最佳药物负载和释放特性的系统。
