• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于治疗应用的生物工程蜘蛛丝和氧化铁纳米粒子的复合球体。

Composite spheres made of bioengineered spider silk and iron oxide nanoparticles for theranostics applications.

机构信息

Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan, Poland.

Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, Poznan, Poland.

出版信息

PLoS One. 2019 Jul 15;14(7):e0219790. doi: 10.1371/journal.pone.0219790. eCollection 2019.

DOI:10.1371/journal.pone.0219790
PMID:31306458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6629150/
Abstract

Bioengineered spider silk is a biomaterial that has exquisite mechanical properties, biocompatibility, and biodegradability. Iron oxide nanoparticles can be applied for the detection and analysis of biomolecules, target drug delivery, as MRI contrast agents and as therapeutic agents for hyperthermia-based cancer treatments. In this study, we investigated three bioengineered silks, MS1, MS2 and EMS2, and their potential to form a composite material with magnetic iron oxide nanoparticles (IONPs). The presence of IONPs did not impede the self-assembly properties of MS1, MS2, and EMS2 silks, and spheres formed. The EMS2 spheres had the highest content of IONPs, and the presence of magnetite IONPs in these carriers was confirmed by several methods such as SEM, EDXS, SQUID, MIP-OES and zeta potential measurement. The interaction of EMS2 and IONPs did not modify the superparamagnetic properties of the IONPs, but it influenced the secondary structure of the spheres. The composite particles exhibited a more than two-fold higher loading efficiency for doxorubicin than the plain EMS2 spheres. For both the EMS2 and EMS2/IONP spheres, the drug revealed a pH-dependent release profile with advantageous kinetics for carriers made of the composite material. The composite spheres can be potentially applied for a combined cancer treatment via hyperthermia and drug delivery.

摘要

生物工程蜘蛛丝是一种具有精致机械性能、生物相容性和可生物降解性的生物材料。氧化铁纳米粒子可用于生物分子的检测和分析、靶向药物输送、磁共振成像对比剂以及基于热疗的癌症治疗的治疗剂。在这项研究中，我们研究了三种生物工程丝，即 MS1、MS2 和 EMS2，以及它们与磁性氧化铁纳米粒子 (IONP) 形成复合材料的潜力。IONP 的存在并不妨碍 MS1、MS2 和 EMS2 丝的自组装特性，并且形成了球体。EMS2 球体具有最高含量的 IONP，并且通过 SEM、EDXS、SQUID、MIP-OES 和 ζ 电位测量等多种方法证实了这些载体中存在磁铁矿 IONP。EMS2 和 IONP 的相互作用并没有改变 IONP 的超顺磁性，但它影响了球体的二级结构。与纯 EMS2 球体相比，复合粒子对阿霉素的负载效率提高了两倍以上。对于 EMS2 和 EMS2/IONP 球体，药物都表现出 pH 依赖性释放特性，对于由复合材料制成的载体来说，这是有利的动力学特性。复合球体可潜在应用于通过热疗和药物输送进行联合癌症治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/1f1af678b3d1/pone.0219790.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/11283c96a33c/pone.0219790.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/414ddf1b170f/pone.0219790.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/039a69c4da50/pone.0219790.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/acb2b2fd73a9/pone.0219790.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/1d60eef90ca9/pone.0219790.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/2fd293d220ba/pone.0219790.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/7b3230ea93a2/pone.0219790.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/1f1af678b3d1/pone.0219790.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/11283c96a33c/pone.0219790.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/414ddf1b170f/pone.0219790.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/039a69c4da50/pone.0219790.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/acb2b2fd73a9/pone.0219790.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/1d60eef90ca9/pone.0219790.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/2fd293d220ba/pone.0219790.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/7b3230ea93a2/pone.0219790.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/449f/6629150/1f1af678b3d1/pone.0219790.g008.jpg

相似文献

1
Composite spheres made of bioengineered spider silk and iron oxide nanoparticles for theranostics applications.用于治疗应用的生物工程蜘蛛丝和氧化铁纳米粒子的复合球体。
PLoS One. 2019 Jul 15;14(7):e0219790. doi: 10.1371/journal.pone.0219790. eCollection 2019.
2
Bioengineering the spider silk sequence to modify its affinity for drugs.对蜘蛛丝序列进行生物工程改造，以改变其对药物的亲和力。
Int J Nanomedicine. 2018 Jul 20;13:4247-4261. doi: 10.2147/IJN.S168081. eCollection 2018.
3
Hyperthermia treatment of cancer cells by the application of targeted silk/iron oxide composite spheres.通过应用靶向性丝/氧化铁复合球体对癌细胞进行热疗。
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111654. doi: 10.1016/j.msec.2020.111654. Epub 2020 Oct 20.
4
Delivery of chemotherapeutics using spheres made of bioengineered spider silks derived from MaSp1 and MaSp2 proteins.使用由 MaSp1 和 MaSp2 蛋白衍生的生物工程蛛丝制成的球体来输送化疗药物。
Nanomedicine (Lond). 2018 Feb;13(4):439-454. doi: 10.2217/nnm-2017-0276. Epub 2018 Jan 17.
5
Functionalized bioengineered spider silk spheres improve nuclease resistance and activity of oligonucleotide therapeutics providing a strategy for cancer treatment.功能化生物工程蜘蛛丝球体提高核酸酶抗性和寡核苷酸疗法的活性，为癌症治疗提供了一种策略。
Acta Biomater. 2017 Sep 1;59:221-233. doi: 10.1016/j.actbio.2017.07.014. Epub 2017 Jul 8.
6
Functionalized spider silk spheres as drug carriers for targeted cancer therapy.功能化蜘蛛丝球体作为靶向癌症治疗的药物载体
Biomacromolecules. 2014 Aug 11;15(8):2971-81. doi: 10.1021/bm500591p. Epub 2014 Jul 7.
7
Blending two bioengineered spider silks to develop cancer targeting spheres.将两种生物工程蜘蛛丝混合以开发癌症靶向球体。
J Mater Chem B. 2017 Apr 28;5(16):3000-3011. doi: 10.1039/c7tb00233e. Epub 2017 Apr 5.
8
The method of purifying bioengineered spider silk determines the silk sphere properties.生物工程蜘蛛丝的提纯方法决定了丝球的性质。
Sci Rep. 2016 Jun 17;6:28106. doi: 10.1038/srep28106.
9
Shape-, size- and structure-controlled synthesis and biocompatibility of iron oxide nanoparticles for magnetic theranostics.用于磁治疗学的磁性氧化铁纳米粒子的形态、尺寸和结构控制合成及生物相容性。
Theranostics. 2018 May 11;8(12):3284-3307. doi: 10.7150/thno.25220. eCollection 2018.
10
2D Superparamagnetic Tantalum Carbide Composite MXenes for Efficient Breast-Cancer Theranostics.二维超顺磁碳化钽复合 MXenes 用于高效乳腺癌诊疗一体化。
Theranostics. 2018 Feb 8;8(6):1648-1664. doi: 10.7150/thno.23369. eCollection 2018.

引用本文的文献

1
Recombinant production of spider silk protein in Physcomitrella photobioreactors.在小立碗藓光生物反应器中重组生产蜘蛛丝蛋白。
Plant Cell Rep. 2025 Apr 26;44(5):103. doi: 10.1007/s00299-025-03485-y.
2
An Insight to Nanoliposomes as Smart Radiopharmaceutical Delivery Tools for Imaging Atherosclerotic Plaques: Positron Emission Tomography Applications.纳米脂质体作为用于成像动脉粥样硬化斑块的智能放射性药物递送工具的见解：正电子发射断层扫描应用
Pharmaceutics. 2025 Feb 12;17(2):240. doi: 10.3390/pharmaceutics17020240.
3
Chemical Conjugation of Iron Oxide Nanoparticles for the Development of Magnetically Directable Silk Particles.

本文引用的文献

1
Blending two bioengineered spider silks to develop cancer targeting spheres.将两种生物工程蜘蛛丝混合以开发癌症靶向球体。
J Mater Chem B. 2017 Apr 28;5(16):3000-3011. doi: 10.1039/c7tb00233e. Epub 2017 Apr 5.
2
Bioengineering the spider silk sequence to modify its affinity for drugs.对蜘蛛丝序列进行生物工程改造，以改变其对药物的亲和力。
Int J Nanomedicine. 2018 Jul 20;13:4247-4261. doi: 10.2147/IJN.S168081. eCollection 2018.
3
Potential use of superparamagnetic iron oxide nanoparticles for in vitro and in vivo bioimaging of human myoblasts.
用于开发磁控丝颗粒的氧化铁纳米颗粒的化学共轭
ACS Appl Mater Interfaces. 2025 Feb 12;17(6):8901-8913. doi: 10.1021/acsami.4c17536. Epub 2025 Feb 3.
4
Review of Spider Silk Applications in Biomedical and Tissue Engineering.蜘蛛丝在生物医学和组织工程中的应用综述
Biomimetics (Basel). 2024 Mar 11;9(3):169. doi: 10.3390/biomimetics9030169.
5
Influence of Spider Silk Protein Structure on Mechanical and Biological Properties for Energetic Material Detection.蜘蛛丝蛋白结构对用于含能材料检测的力学和生物学性能的影响。
Molecules. 2024 Feb 27;29(5):1025. doi: 10.3390/molecules29051025.
6
Magnetic Hydroxyapatite Nanoparticles in Regenerative Medicine and Nanomedicine.再生医学与纳米医学中的磁性羟基磷灰石纳米颗粒
Int J Mol Sci. 2024 Feb 28;25(5):2809. doi: 10.3390/ijms25052809.
7
Factors Influencing Properties of Spider Silk Coatings and Their Interactions within a Biological Environment.影响蜘蛛丝涂层性能及其在生物环境中相互作用的因素。
J Funct Biomater. 2023 Aug 19;14(8):434. doi: 10.3390/jfb14080434.
8
Delivering on the promise of recombinant silk-inspired proteins for drug delivery.兑现基于丝蛋白的重组蛋白类药物传递系统的承诺。
Adv Drug Deliv Rev. 2023 Jan;192:114622. doi: 10.1016/j.addr.2022.114622. Epub 2022 Nov 19.
9
SPIONs Conjugate Supported Anticancer Drug Doxorubicin's Delivery: Current Status, Challenges, and Prospects.超顺磁性氧化铁纳米颗粒缀合的抗癌药物阿霉素递送：现状、挑战与前景
Nanomaterials (Basel). 2022 Oct 20;12(20):3686. doi: 10.3390/nano12203686.
10
Radiolabeled nanomaterials for biomedical applications: radiopharmacy in the era of nanotechnology.用于生物医学应用的放射性标记纳米材料：纳米技术时代的放射性药物学。
EJNMMI Radiopharm Chem. 2022 Apr 25;7(1):8. doi: 10.1186/s41181-022-00161-4.
超顺磁性氧化铁纳米粒子在人肌母细胞体外和体内生物成像中的潜在应用。
Sci Rep. 2018 Feb 27;8(1):3682. doi: 10.1038/s41598-018-22018-0.
4
Delivery of chemotherapeutics using spheres made of bioengineered spider silks derived from MaSp1 and MaSp2 proteins.使用由 MaSp1 和 MaSp2 蛋白衍生的生物工程蛛丝制成的球体来输送化疗药物。
Nanomedicine (Lond). 2018 Feb;13(4):439-454. doi: 10.2217/nnm-2017-0276. Epub 2018 Jan 17.
5
The method of purifying bioengineered spider silk determines the silk sphere properties.生物工程蜘蛛丝的提纯方法决定了丝球的性质。
Sci Rep. 2016 Jun 17;6:28106. doi: 10.1038/srep28106.
6
A composited PEG-silk hydrogel combining with polymeric particles delivering rhBMP-2 for bone regeneration.一种复合聚乙二醇-丝水凝胶与递送重组人骨形态发生蛋白-2的聚合物颗粒相结合用于骨再生。
Mater Sci Eng C Mater Biol Appl. 2016 Aug 1;65:221-31. doi: 10.1016/j.msec.2016.04.043. Epub 2016 Apr 14.
7
Study of magnetic silk fibroin nanoparticles for massage-like transdermal drug delivery.用于类按摩透皮给药的磁性丝素纳米颗粒研究
Int J Nanomedicine. 2015 Jul 21;10:4639-51. doi: 10.2147/IJN.S85999. eCollection 2015.
8
Enhanced cellular uptake of engineered spider silk particles.增强型工程蜘蛛丝颗粒的细胞摄取。
Biomater Sci. 2015 Mar;3(3):543-51. doi: 10.1039/c4bm00401a. Epub 2015 Jan 13.
9
Silk as an innovative biomaterial for cancer therapy.丝绸作为一种用于癌症治疗的创新生物材料。
Rep Pract Oncol Radiother. 2014 Dec 18;20(2):87-98. doi: 10.1016/j.rpor.2014.11.010. eCollection 2015 Mar-Apr.
10
Biomimetic magnetic silk scaffolds.仿生磁性丝支架
ACS Appl Mater Interfaces. 2015 Mar 25;7(11):6282-92. doi: 10.1021/acsami.5b00529. Epub 2015 Mar 10.