Hu Kai, Xie Xiujing, Wang Rensheng, Wu Fang, Zhang Yong
Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
Department of Ultrasonic Medicine, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
Oncol Lett. 2017 Dec;14(6):7681-7686. doi: 10.3892/ol.2017.7209. Epub 2017 Oct 18.
Vascular endothelial growth factor () and its receptor, , serve a critical role in angiogenesis and lymphangiogenesis, which are involved in the initiation and progression of malignancies. Specific single nucleotide polymorphisms of and have been shown to modulate gene expression and influence malignancy aggressiveness. The aim of the present study was to determine whether the rs2071559 (T/C) polymorphism is associated with the risk of developing nasopharyngeal carcinoma (NPC) and the aggressiveness of NPC in a southern Chinese population. A case-control study comprising 171 NPC patients and 184 healthy individuals was performed. Genotyping of the rs2071559 polymorphism was performed by quantitative polymerase chain reaction using TaqMan probes. Genotype and allele distribution of the rs2071559 polymorphism was not associated with the risk of NPC following adjustment for age, sex and ethnicity by multivariate logistic regression analyses. Regional lymph node metastasis was significantly correlated with the rs2071559 C allele and the related genotypes (OR 0.402, 95% CI 0.193-0.835, P=0.016; and OR 0.347, 95% CI 0.145-0.829, P=0.024, respectively). No correlations between genotype or allele distribution and the primary tumor size, distant metastasis, clinical stage, or histological type were observed. The rs2071559 polymorphism was shown to have an association with lymphatic metastasis in patients with NPC; however, the precise molecular mechanism should be elucidated in additional studies.
血管内皮生长因子（VEGF）及其受体在血管生成和淋巴管生成中起关键作用，而血管生成和淋巴管生成与恶性肿瘤的发生和发展有关。VEGF和其受体的特定单核苷酸多态性已被证明可调节基因表达并影响恶性肿瘤的侵袭性。本研究的目的是确定VEGF rs2071559（T/C）多态性是否与中国南方人群患鼻咽癌（NPC）的风险以及NPC的侵袭性相关。进行了一项病例对照研究，包括171例NPC患者和184名健康个体。使用TaqMan探针通过定量聚合酶链反应对rs2071559多态性进行基因分型。通过多因素逻辑回归分析对年龄、性别和种族进行校正后，rs2071559多态性的基因型和等位基因分布与NPC风险无关。区域淋巴结转移与rs2071559 C等位基因及相关基因型显著相关（分别为OR 0.402，95%CI 0.193 - 0.835，P = 0.016；以及OR 0.347，95%CI 0.145 - 0.829，P = 0.024）。未观察到基因型或等位基因分布与原发肿瘤大小、远处转移、临床分期或组织学类型之间的相关性。rs2071559多态性与NPC患者的淋巴转移有关；然而，确切的分子机制应在进一步研究中阐明。
