OMFS IMPATH research group, Department Imaging & Pathology, Faculty of Medicine, KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Leuven, Belgium.
State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, China.
Clin Oral Investig. 2018 Mar;22(2):633-640. doi: 10.1007/s00784-018-2337-x. Epub 2018 Jan 17.
The objective of the study was to conduct a systematic review of the literature assessing potential salivary biomarkers of oral cancer and pre-cancer and discuss emerging issues and challenges in relation to oral cancer and pre-cancer diagnostics.
Search for articles involved the Medline, PubMed, and EMBASE. Specific terms were used from January 1995 to March 2017 by three experts.
This search collected 270 articles, of which 105 articles such as reviews, case reports, news, letter to editor, etc. in first round and 117 articles such as publications in other languages than English, non-human studies, etc. were excluded. The remaining 48 articles considered analyzing whole saliva as well as specific gland saliva. Thirty-one studies considered oral stimuli such as eating, drinking, and oral hygiene practices for varied periods of time prior to sample collection. The time of collection of saliva was morning in most studies, but the exact time of collection was not mentioned. Three studies showed to have evaluated the whole saliva without centrifugation. Two-dimensional gel electrophoresis and tandem mass spectrometry were the most commonly used methods. Most of the potential salivary biomarkers of oral cancer are salivary proteins.
Combination approach of salivary biomarkers could be used as screening tool to improve early detection and diagnostic precision of oral pre-cancer and cancer.
The current findings are of importance for clinicians and researchers to mitigate the challenges in salivary-based diagnosis of oral cancer and to evaluate reliable, specific, and sensitive salivary biomarkers for oral pre-cancer and cancer diagnosis.
本研究旨在对评估口腔癌和癌前病变潜在唾液生物标志物的文献进行系统综述，并讨论与口腔癌和癌前病变诊断相关的新出现问题和挑战。
通过三位专家，使用特定术语对 1995 年 1 月至 2017 年 3 月期间的 Medline、PubMed 和 EMBASE 中的文章进行检索。
该检索共收集到 270 篇文章，其中 105 篇文章（如综述、病例报告、新闻、给编辑的信等）在第一轮被排除，117 篇文章（如英语以外语言的出版物、非人类研究等）被排除。剩余的 48 篇文章考虑了全唾液和特定腺体唾液的分析。31 项研究考虑了口腔刺激因素，如在采集样本前进食、饮水和口腔卫生习惯等，持续不同时间。大多数研究在早上采集唾液，但未提及确切的采集时间。三项研究显示评估了未经离心的全唾液。二维凝胶电泳和串联质谱是最常用的方法。大多数口腔癌的潜在唾液生物标志物是唾液蛋白。
唾液生物标志物的联合方法可作为筛查工具，提高口腔癌前病变和癌症的早期检测和诊断精度。
目前的研究结果对临床医生和研究人员具有重要意义，有助于解决基于唾液的口腔癌诊断中的挑战，并评估用于口腔癌前病变和癌症诊断的可靠、特异和敏感的唾液生物标志物。
