Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain.
Mar Drugs. 2012 Dec 10;10(12):2766-81. doi: 10.3390/md10122766.
Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC₅₀ = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC₅₀ = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC₅₀ = 0.93 mg/mL dry weight), followed by F. spiralis (IC₅₀ = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process.
四种褐藻（皱枝马尾藻（Withering）M. Roberts、长松萝（Hudson）Papenfuss、网地藻（Linnaeus）M. Roberts 和螺旋藻（Linnaeus））的纯化岩藻多酚提取物通过 HPLC-DAD-ESI-MSn 进行了表征。鉴定了岩藻福酚醇、岩藻二磷酚醇、岩藻三磷酚醇、7-漆酚、岩藻福酚和贝克醇/迪考醇。还评估了提取物的抗氧化活性和透明质酸酶（HAase）抑制能力。建立了提取物活性与其化学成分之间的相关性。螺旋藻，具有较高分子量岩藻多酚的物种，通常表现出最强的脂质过氧化抑制活性（IC₅₀=2.32mg/mL 干重）和最强的 HAase 抑制能力（IC₅₀=0.73mg/mL 干重）。至于超氧自由基清除，网地藻是最有效的物种（IC₅₀=0.93mg/mL 干重），其次是螺旋藻（IC₅₀=1.30mg/mL 干重）。这些结果表明，纯化的岩藻多酚提取物具有预防和减缓皮肤衰老过程的强大能力，这主要与自由基损伤和透明质酸浓度降低有关，这是衰老过程的特征。
