从埃及患病本地鸡和鸽子中分离出的具有动物源潜力的沙门氏菌的抗生素耐药谱和遗传多样性。
Antibiogram and genetic diversity of Salmonella enterica with zoonotic potential isolated from morbid native chickens and pigeons in Egypt.
机构信息
Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Sadat City University, Sadat, Egypt.
Hygiene and Preventive Medicine (Zoonoses) Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.
出版信息
J Appl Microbiol. 2018 May;124(5):1265-1273. doi: 10.1111/jam.13697. Epub 2018 Feb 9.
AIMS
This study investigated the possible role of Fayoumi chickens and pigeons in the transmission of multidrug resistant (MDR) Salmonella with zoonotic potential.
METHODS AND RESULTS
Morbid Fayoumi chickens (70) and pigeons (30) were examined to detect the prevalence and antibiotic resistance of Salmonella and to detect and sequence sodC-1 gene as a zoonotic and phylogenetic marker. Salmonella isolates were detected in 14·3 and 20% of the examined Fayoumi chickens and pigeons, respectively. Salomonella subspecies salamae (43·8%) and S. subspecies enterica serovar Bukuru (31·3%) were the most prevalent isolates. All tested Salmonella isolates were MDR to at least five classes of antibiotics. S. salamae and S. Bukuru isolates that carried bla , qnrS, aadA2 and floR genes expressed phenotypic resistance to cefotaxime, ciprofloxacin, streptomycin and chloramphenicol, respectively. The aacC gene was detected in one of each S. salamae and S. Bukuru isolate, although only the S. Bukuru isolate showed phenotypic resistance to gentamicin. The sequence analysis of the sodC-1 gene from Salmonella isolates showed clear inter- and intra-subspecies phylogenetic segregation.
CONCLUSIONS
Fayoumi chickens and pigeons could act as reservoirs of MDR Salmonella.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study highlights the possible emergence of Salmonella subspecies salamae with zoonotic potential.
目的
本研究旨在探讨法尤姆鸡和鸽子在传播具有动物源性的多重耐药（MDR）沙门氏菌方面的可能作用。
方法和结果
对患有疾病的法尤姆鸡（70 只）和鸽子（30 只）进行检查，以检测沙门氏菌的流行率和抗生素耐药性，并检测和测序 sodC-1 基因作为动物源性和系统发育标记。分别在 14.3%和 20%的检查法尤姆鸡和鸽子中检测到沙门氏菌。最常见的分离株是沙门氏菌亚种 salamae（43.8%）和 S. 亚种 enterica 血清型 Bukuru（31.3%）。所有测试的沙门氏菌分离株对至少五类抗生素均具有耐药性。携带 bla、qnrS、aadA2 和 floR 基因的 S. salamae 和 S. Bukuru 分离株分别表现出对头孢噻肟、环丙沙星、链霉素和氯霉素的表型耐药性。在每一个 S. salamae 和 S. Bukuru 分离株中都检测到了 aacC 基因，尽管只有 S. Bukuru 分离株对庆大霉素表现出表型耐药性。沙门氏菌分离株 sodC-1 基因的序列分析显示出明显的种间和种内系统发育分离。
结论
法尤姆鸡和鸽子可能是 MDR 沙门氏菌的储存宿主。
研究意义和影响
本研究强调了具有动物源性的沙门氏菌亚种 salamae 的出现的可能性。
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