Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience), C/Faraday 9, Ciudad Universitaria de Cantoblanco, Madrid 28049, Spain.
Bioinspir Biomim. 2018 Feb 27;13(2):026011. doi: 10.1088/1748-3190/aaa903.
The rapid emergence of antibiotic resistant bacteria has prompted the need for radically different approaches to combat bacterial infections. Among these, bioinspired surface topographies have emerged as an effective sustainable strategy to deter bacterial infection. This study demonstrates the bactericidal activity and cytocompatibility of the moth-eye mimetic topography produced by thermal polymer nanoimprinting. The moth-eye topography was found to have bactericidal capabilities against Gram negative and Gram positive bacteria. Electron microscopy imaging revealed the bactericidal effect caused by mechanical rupture of the bacteria wall inflicted by the topography on the adhered cells. The cytocompatibility of the surfaces was evidenced by assessing the proliferation and morphology of keratinocytes cultured on the nanotopography. The technology meets important needs in medical implant technology for materials that not only have good biocompatibility but also antibacterial properties for reducing the risk of infections and related health complications.
抗生素耐药菌的迅速出现促使人们需要采取截然不同的方法来对抗细菌感染。在这些方法中，受生物启发的表面形貌已成为一种有效且可持续的策略，可以防止细菌感染。本研究展示了通过热聚合纳米压印技术制备的模拟蛾眼形貌的杀菌活性和细胞相容性。研究发现，蛾眼形貌对革兰氏阴性菌和革兰氏阳性菌均具有杀菌能力。电子显微镜成像揭示了这种形貌对附着细胞造成的细菌细胞壁机械破裂导致的杀菌作用。通过评估培养在纳米形貌上的角质形成细胞的增殖和形态，证明了表面的细胞相容性。该技术满足了医疗植入物技术中对材料的重要需求，这些材料不仅具有良好的生物相容性，而且具有抗菌性能，可以降低感染和相关健康并发症的风险。
