Minoura Kiyoshi, Yamada Miho, Mizoguchi Takashi, Kaneko Toshihiro, Nishiyama Kyoko, Ozminskyj Mari, Koshizuka Tetsuo, Wada Ikuo, Suzutani Tatsuo
LCD Technology Development Center, Development Group, Display Device Company, Sharp Corporation, Tenri, Nara, Japan.
New Business Promotion Center, Development Group, Display Device Company, Sharp Corporation, Tenri, Nara, Japan.
PLoS One. 2017 Sep 21;12(9):e0185366. doi: 10.1371/journal.pone.0185366. eCollection 2017.
The antibacterial effect of a nanostructured film, known as "moth-eye film," was investigated. The moth-eye film has artificially formed nano-pillars, consisting of hydrophilic resin with urethane acrylate and polyethylene glycol (PEG) derivatives, all over its surface that replicates a moth's eye. Experiments were performed to compare the moth-eye film with a flat-surfaced film produced from the same materials. The JIS Z2801 film-covering method revealed that the two films produced a decrease in Staphylococcus aureus and Esherichia coli titers of over 5 and 3 logs, respectively. There was no marked difference in the antibacterial effects of the two surfaces. However, the antibacterial effects were reduced by immersion of the films in water. These results indicated that a soluble component(s) of the resin possessed the antibacterial activity, and this component was identified as PEG derivatives by time-of-flight secondary ion mass spectrometry (TOF-SIMS) and Fourier transform infrared spectroscopy (FT-IR). When a small volume of bacterial suspension was dropped on the films as an airborne droplet model, both films showed antibacterial effects, but that of the moth-eye film was more potent. It was considered that the moth-eye structure allowed the bacteria-loaded droplet to spread and allow greater contact between the bacteria and the film surface, resulting in strong adherence of the bacteria to the film and synergistically enhanced bactericidal activity with chemical components. The antibacterial effect of the moth-eye film has been thus confirmed under a bacterial droplet model, and it appears attractive due to its antibacterial ability, which is considered to result not only from its chemical make-up but also from physical adherence.
研究了一种名为“蛾眼膜”的纳米结构薄膜的抗菌效果。蛾眼膜在其整个表面人工形成了纳米柱，这些纳米柱由含有聚氨酯丙烯酸酯和亲水性树脂以及聚乙二醇（PEG）衍生物组成，其表面模仿了蛾眼。进行了实验，将蛾眼膜与由相同材料制成的平面薄膜进行比较。JIS Z2801薄膜覆盖法显示，这两种薄膜分别使金黄色葡萄球菌和大肠杆菌的滴度降低了超过5个对数和3个对数。两种表面的抗菌效果没有显著差异。然而，将薄膜浸入水中后，抗菌效果会降低。这些结果表明，树脂的一种可溶性成分具有抗菌活性，通过飞行时间二次离子质谱（TOF-SIMS）和傅里叶变换红外光谱（FT-IR）鉴定该成分是PEG衍生物。当将少量细菌悬浮液作为空气传播液滴模型滴在薄膜上时，两种薄膜都显示出抗菌效果，但蛾眼膜的抗菌效果更强。据认为，蛾眼结构使载有细菌的液滴扩散，使细菌与薄膜表面有更大的接触，导致细菌强烈粘附在薄膜上，并与化学成分协同增强杀菌活性。因此，在细菌液滴模型下证实了蛾眼膜的抗菌效果，并且由于其抗菌能力，它显得很有吸引力，这种抗菌能力被认为不仅源于其化学组成，还源于物理粘附。
