School of Food Science and Engineering, South China University of Technology, 381Wushan Road, Guangzhou, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou, China.
School of Food Science and Engineering, South China University of Technology, 381Wushan Road, Guangzhou, China.
Carbohydr Polym. 2018 Mar 1;183:230-239. doi: 10.1016/j.carbpol.2017.12.048. Epub 2017 Dec 19.
The aim of the present study was to investigate structural characteristic and in vitro fermentation of a novel polysaccharide named ST-P2 from Sargassum thunbergii by human fecal inoculums, and its impact on human colonic microbiota. The results showed that ST-P2 was homogeneous with molecular weight of 48,788 Da, and consisted of arabinose, galactose, glucose, xylose, and mannose. The main linkage types were identified as (1 → 5)-α-L-Araf, (1 → 3)-α-L-Manp, (1 → 3,6)-β-D-Galp, (1 → 6)-α-D-Glcp, and (1 → 3)-β-D-Xylp, respectively. After 48 h fermentation, 67.83 ± 1.15% of total carbohydrate was utilized by colonic microbiota. The pH value in the fecal culture significantly decreased from 6.09 ± 0.11 to 4.70 ± 0.04. The concentrations of total short chain fatty acids, acetic, propionic, n-butyric and n-valeric acids significantly increased compared to the blank. ST-P2 could remarkably modulate the composition and abundance of beneficial microbiota. These results suggest that ST-P2 could potentially be a functional food aimed at promoting the gut health.
本研究旨在通过人体粪便接种物研究一种新型褐藻多糖 ST-P2 的结构特征和体外发酵特性，及其对人体结肠微生物群的影响。结果表明，ST-P2 均一分子量为 48788 Da，由阿拉伯糖、半乳糖、葡萄糖、木糖和甘露糖组成。主要连接类型分别鉴定为（1→5）-α-L-阿拉伯呋喃糖、（1→3）-α-L-甘露吡喃糖、（1→3,6）-β-D-半乳糖吡喃糖、（1→6）-α-D-葡萄糖吡喃糖和（1→3）-β-D-木吡喃糖。发酵 48 h 后，结肠微生物群利用总碳水化合物的 67.83±1.15%。粪便培养物的 pH 值从 6.09±0.11 显著降低至 4.70±0.04。与空白组相比，总短链脂肪酸、乙酸、丙酸、丁酸和异丁酸的浓度显著增加。ST-P2 可以显著调节有益微生物群的组成和丰度。这些结果表明，ST-P2 可能是一种具有促进肠道健康功能的功能性食品。
