Yu C L, Haskard D O, Cavender D, Johnson A R, Ziff M
Clin Exp Immunol. 1985 Dec;62(3):554-60.
Binding of lymphocytes to human umbilical vein endothelial cells (EC) was quantitated by measuring adhesion of 51Cr labelled lymphocytes to endothelial cell monolayers and rosette formation between lymphocytes and EC in suspension. Mitogen stimulated human peripheral blood mononuclear cell culture supernatants and mixed lymphocyte reaction supernatants enhanced the binding of T lymphocytes to EC monolayers or suspensions preincubated with such supernatants. The active component of these supernatants appeared to be gamma interferon (IFN-gamma) since culture supernatants lost activity after heating at 56 degrees C for 60 min, exposure to pH 2.0 or treatment with anti-IFN-gamma. In addition, purified IFN-gamma increased the binding of T lymphocytes to EC (T-EC). This occurred in a concentration dependent manner when IFN-gamma was preincubated with EC but not with lymphocytes. While the optimum concentration of IFN-gamma was 250 u/ml, a significant enhancement was seen with as little as 10 u/ml. These findings suggest that IFN-gamma may play a part in the emigration of lymphocytes to perivascular chronic inflammatory sites by augmenting the adhesion of lymphocytes to the endothelium of small blood vessels.
通过测量51Cr标记的淋巴细胞与内皮细胞单层的黏附以及悬浮状态下淋巴细胞与内皮细胞之间的玫瑰花结形成，对淋巴细胞与人脐静脉内皮细胞（EC）的结合进行定量。丝裂原刺激的人外周血单个核细胞培养上清液和混合淋巴细胞反应上清液增强了T淋巴细胞与预先用此类上清液孵育的内皮细胞单层或悬浮液的结合。这些上清液的活性成分似乎是γ干扰素（IFN-γ），因为培养上清液在56℃加热60分钟、暴露于pH 2.0或用抗IFN-γ处理后失去活性。此外，纯化的IFN-γ增加了T淋巴细胞与内皮细胞（T-EC）的结合。当IFN-γ与内皮细胞而非淋巴细胞预先孵育时，这种结合以浓度依赖的方式发生。虽然IFN-γ的最佳浓度为250 u/ml，但低至10 u/ml时也能观察到显著增强。这些发现表明，IFN-γ可能通过增强淋巴细胞与小血管内皮的黏附，在淋巴细胞向血管周围慢性炎症部位的迁移中发挥作用。
