Department of Periodontology, Endodontology and Cariology, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056, Basel, Switzerland.
Clinic of Preventive Dentistry and Oral Microbiology, University Center for Dental Medicine, University of Basel, Basel, Switzerland.
Clin Oral Investig. 2018 Jul;22(6):2141-2147. doi: 10.1007/s00784-017-2305-x. Epub 2018 Jan 22.
The objective of this study was to investigate the influence of different oscillation frequencies of three powered toothbrushes with side-to-side action for noncontact biofilm removal in an artificial interdental space model.
A three-species biofilm (Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis) was formed in vitro on protein-coated titanium disks using a flow chamber system combined with a static biofilm growth model. The oscillation frequencies of three commercial side-to-side toothbrushes were evaluated by means of a dose response. The frequency was decreased in steps (100, 85, 70, 55, and 40%). Subsequently, the biofilm-coated substrates were exposed to the side-to-side toothbrushes. The biofilm volumes were measured using volumetric analyses (Imaris 8.1.2) with confocal laser scanning microscope images (Zeiss LSM700).
Compared to maximum oscillation frequency (100%), lower oscillation frequencies (up to 40%) resulted in reduced median percentages of biofilm reduction (median biofilm reduction up to 53% for maximum oscillation frequency, and up to 13% for 40% oscillation frequency) (p ≥ 0.03). In addition, decreasing the oscillation frequencies of the side-to-side toothbrushes showed an enhanced variety in the results of repeated experiments.
The oscillation frequency of the tested side-to-side toothbrushes affected the biofilm reduction in an interdental space model.
Within a toothbrush, higher oscillation frequencies may lead to beneficial effects on interdental biofilm removal by noncontact brushing.
本研究旨在探讨三种具有侧向运动的动力牙刷在非接触式牙间隙模型中对生物膜去除的不同振荡频率的影响。
使用结合静态生物膜生长模型的流动室系统，在涂有蛋白质的钛盘上体外形成三物种生物膜（牙龈卟啉单胞菌、核梭杆菌和血链球菌）。通过剂量反应评估三种市售侧向牙刷的振荡频率。频率以步长降低（100、85、70、55 和 40%）。随后，将涂有生物膜的基底暴露于侧向牙刷。使用共聚焦激光扫描显微镜图像（Zeiss LSM700）的体积分析（Imaris 8.1.2）测量生物膜体积。
与最大振荡频率（100%）相比，较低的振荡频率（最高 40%）导致生物膜减少的中位数百分比降低（最大振荡频率的中位数生物膜减少高达 53%，40%振荡频率的中位数生物膜减少高达 13%）（p≥0.03）。此外，降低侧向牙刷的振荡频率显示出增强了重复实验结果的多样性。
测试的侧向牙刷的振荡频率会影响牙间隙模型中的生物膜减少。
在牙刷内，较高的振荡频率可能会通过非接触式刷牙对牙间隙生物膜去除产生有益效果。
