Kılıç Murat Özgür, Aynekin Büşra, Bozer Mikdat, Kara Adem, Haltaş Hacer, İçen Duygu, Demircan Kadir
Department of General Surgery, Numune Training and Research Hospital, Ankara, Turkey.
Department of Medical Genetics, Faculty of Medicine, Turgut Ozal University, Ankara, Turkey.
Prz Gastroenterol. 2017;12(4):262-270. doi: 10.5114/pg.2017.72101. Epub 2017 Dec 14.
Despite recent diagnostic and therapeutic improvements, pancreas cancer remains one of the highly lethal cancers. The extracellular matrix (ECM) is a physiological barrier that limits the spread of cancer cells into surrounding tissues and distant organs. Disintegrin and metalloprotease with thrombospondin motifs (ADAMTS) is a family of 19 proteases, which is involved in various biological processes such as ECM remodelling and anti-angiogenesis.
To investigate the expression of ADAMTS1, 8, 9, and 18 proteinases in pancreas adenocarcinoma and its nodal metastasis.
The immunostaining status of ADAMTS1, 8, 9, and 18 were investigated in formalin-fixed paraffin-embedded samples of 25 patients who underwent pancreaticoduodenectomy for an adenocarcinoma located at the head of the pancreas.
In semi-quantitive grading pathologically, ADAMTS1, 8, 9, and 18 were found to be highly stained in all cancerous pancreas samples compared with normal pancreas. In addition, the immune positivity of ADAMTS1, 9, and 18 was found to be higher in metastatic lymph nodes than in non-metastatic lymph tissue. Tumour size was correlated with ADAMTS9 and 18 expressions in cancerous pancreas.
According to the data obtained from the study, we suggest that these four ADAMTSs may have significant roles in the tumorigenesis and nodal spread of pancreas adenocarcinoma.
尽管近期在诊断和治疗方面有所改进，但胰腺癌仍然是致死率极高的癌症之一。细胞外基质（ECM）是一种生理屏障，可限制癌细胞扩散至周围组织和远处器官。含血小板反应蛋白基序的解聚素和金属蛋白酶（ADAMTS）是一个由19种蛋白酶组成的家族，参与细胞外基质重塑和抗血管生成等多种生物学过程。
研究ADAMTS1、8、9和18蛋白酶在胰腺腺癌及其淋巴结转移中的表达情况。
对25例行胰十二指肠切除术的胰腺头部腺癌患者的福尔马林固定石蜡包埋样本进行ADAMTS1、8、9和18的免疫染色检测。
在病理半定量分级中，与正常胰腺相比，所有癌性胰腺样本中ADAMTS1、8、9和18均呈高染色。此外，发现ADAMTS1、9和18在转移性淋巴结中的免疫阳性率高于非转移性淋巴组织。肿瘤大小与癌性胰腺中ADAMTS9和18的表达相关。
根据本研究获得的数据，我们认为这四种ADAMTS可能在胰腺腺癌的肿瘤发生和淋巴结转移中起重要作用。
