Erdmann D, Schütt C
Allerg Immunol (Leipz). 1985;31(4):259-64.
To detect intracytoplasmic antibodies after PWM-stimulation of peripheral mononuclear cells we used class specific antisera against IgG, A and M and ascertained the whole number of cIg+-cells by addition. The number of cIg+-cells after culturing 7 days without mitogen was below 1%, with PWM-stimulation 14,7% +/- 8,5 (n = 29, blood donors). We discussed the requirements for the culture conditions and for the immunofluorescence detection.
为检测经PWM刺激的外周血单个核细胞中的胞浆内抗体,我们使用了针对IgG、A和M的类特异性抗血清,并通过相加确定cIg⁺细胞的总数。在无丝裂原培养7天后,cIg⁺细胞数量低于1%,经PWM刺激后为14.7%±8.5(n = 29,献血者)。我们讨论了培养条件和免疫荧光检测的要求。